Related Experiment Video
Updated: Sep 30, 2025
07:22
Basophil Activation Test for Allergy Diagnosis
Published on: May 31, 2021
8.6K
IFN-γ Induces PD-L1 Expression in Primed Human Basophils
Srinivasa Reddy Bonam1, Camille Chauvin1, Mano J Mathew2
1Institut National de la Santé et de la Recherche Médicale, Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, 75006 Paris, France.
Cells
|March 10, 2022
Summary
Interferon-gamma (IFN-γ) induces Programmed death-ligand 1 (PD-L1) in human basophils, but only when they are primed by Interleukin-3 (IL-3). This priming is crucial for IFN-γ to trigger PD-L1 expression.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Programmed death-ligand 1 (PD-L1) is vital for immune tolerance and cancer immune evasion.
- While stimuli for PD-L1 induction in many innate cells are known, data on human basophils are limited.
Purpose of the Study:
- To identify stimuli that induce PD-L1 expression in human basophils.
- To elucidate the role of cytokine priming in IFN-γ-induced PD-L1 expression on basophils.
Main Methods:
- Investigated IFN-γ-induced PD-L1 expression in human basophils.
- Assessed the necessity of priming by IL-3, GM-CSF, and TSLP for IFN-γ-induced PD-L1.
- Analyzed microarray data of IL-3-treated basophils to understand molecular mechanisms.
Main Results:
- Identified IFN-γ as an inducer of PD-L1 expression in human basophils.
- Demonstrated that IL-3 priming is indispensable for IFN-γ-induced PD-L1 expression.
- Showed that priming with GM-CSF or TSLP was dispensable for this effect.
- Microarray analysis revealed IL-3 upregulates IFNGR2 and CD274 in basophils.
Conclusions:
- IL-3 priming is essential for IFN-γ to induce PD-L1 expression in human basophils.
- IL-3 enhances IFNGR2 and CD274 expression, providing a mechanistic basis for its priming effect.
