A method for a quantitative determination of changes in tissue volume as a result of perfusion fixation

A method for quantifying changes in volume of tissue as a result of perfusion fixation is described. This is achieved by setting standards into the tissue in vivo and in situ by applying dyes to the intact tissue at known distances. Immediately after perfusion the distances of the dyes are macroscopically measured and the comparison between the corresponding distances before and after perfusion enables a quantification of the alteration of the tissue. The total error of this method is approximately 0.5-0.8% (linear).