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Related Concept Videos

Tight Junctions01:29

Tight Junctions

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Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
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Overview of Cell-Cell Junctions01:14

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The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another  cell. These cell junctions are classified  into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight...
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Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

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A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
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Intracellular Signaling Affects Focal Adhesions01:17

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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
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Anchoring Junctions01:03

Anchoring Junctions

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Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
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The Role of Actin and Myosin in Non-muscle Cells01:10

The Role of Actin and Myosin in Non-muscle Cells

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Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They  are held...
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Author's Reply to Yang and Wang: "Personalized Medicine in Cystic Fibrosis: Characterization of Eight Rare CFTR Variants in Intestinal Organoids and Cellular Models".

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Cystic fibrosis as a paradigmatic disease in bringing science to the bedside.

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Additive Potentiation of R334W-CFTR Function by Novel Small Molecules.

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Related Experiment Video

Updated: Sep 30, 2025

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
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In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

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CFTR, Cell Junctions and the Cytoskeleton.

Ines Pankonien1, Margarida C Quaresma1, Cláudia S Rodrigues1

  • 1BioISI-Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, 1749-016 Lisboa, Portugal.

International Journal of Molecular Sciences
|March 10, 2022
PubMed
Summary
This summary is machine-generated.

Cystic Fibrosis (CF) arises from CF transmembrane conductance regulator (CFTR) mutations, impacting ion transport and mucus. Emerging research reveals CFTR

Keywords:
cell polarizationcystic fibrosisepithelial differentiationepithelial regenerationepithelial-mesenchymal transitionextracellular matrix

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Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Physiology

Background:

  • Cystic Fibrosis (CF) is a multi-organ disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene.
  • CFTR protein dysfunction leads to impaired ion transport, resulting in thick mucus accumulation in organs like the lungs and pancreas.
  • Beyond ion transport, CFTR is increasingly linked to cellular structural functions.

Purpose of the Study:

  • To review recent literature (last 10 years) on CFTR's role in epithelial cell biology.
  • To explore CFTR's involvement in actin cytoskeleton organization.
  • To investigate CFTR's connection to epithelial cell junctions and extracellular matrix (ECM) proteins.

Main Methods:

  • Literature review focusing on studies published within the last decade.
  • Analysis of research investigating CFTR's interactions with cytoskeletal and junctional proteins.
  • Synthesis of findings related to CFTR's influence on epithelial polarity.

Main Results:

  • Evidence suggests CFTR influences actin cytoskeleton organization.
  • CFTR plays a role in the formation and maintenance of epithelial cell junctions.
  • CFTR is implicated in maintaining epithelial apical basolateral polarity through interactions with ECM proteins.

Conclusions:

  • CFTR's functions extend beyond ion channel activity.
  • CFTR is crucial for maintaining epithelial structure and polarity.
  • Further research into CFTR's non-canonical roles is warranted for a comprehensive understanding of CF and potential therapeutic strategies.