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Transcriptome Profiling and Functional Validation of RING-Type E3 Ligases in Halophyte Sesuvium verrucosum under
Fayas Thayale Purayil1,2, Naganeeswaran Sudalaimuthuasari1, Ling Li1
1Khalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al-Ain P.O. Box 15551, United Arab Emirates.
International Journal of Molecular Sciences
|March 10, 2022
Summary
Sesuvium verrucosum E3 ligases are key to salt tolerance. Nine identified E3 ligase genes conferred salt tolerance in yeast, offering potential for developing stress-tolerant crops.
Area of Science:
- Plant biology
- Molecular biology
- Genomics
Background:
- Plants possess molecular mechanisms to adapt to environmental challenges like abiotic stress.
- The Aizoaceae family, including Sesuvium verrucosum, exhibits remarkable adaptation to arid and saline environments.
- Ubiquitin-mediated protein degradation, involving E3 ligases, is crucial for plant stress tolerance.
Purpose of the Study:
- To characterize E3 ligases in the salt-tolerant halophyte Sesuvium verrucosum.
- To investigate the role of E3 ligases in plant response to salinity stress.
- To provide a foundation for developing salt-tolerant crops.
Main Methods:
- De novo transcriptome assembly of S. verrucosum roots under salinity stress.
- Positive selection analysis and BUSCO analysis for transcriptome completeness.
- Identification and differential expression analysis of E3 ligase transcripts.
- Functional validation of selected E3 ligase genes using yeast assays.
Main Results:
- A comprehensive transcriptome of S. verrucosum was generated, revealing 131,454 transcripts.
- 101 gene families under positive selection were enriched for stress responses and protein catabolism.
- 433 E3 ligase transcripts were identified, with single RING-type E3 ligases being predominant.
- Nine out of twelve tested E3 ligase genes conferred significant salt tolerance in transgenic yeast.
Conclusions:
- Single RING-type E3 ligases in S. verrucosum are abundant and play a critical role in salinity stress response.
- Functional assays confirm the involvement of specific E3 ligases in conferring salt tolerance.
- These findings support the potential application of S. verrucosum E3 ligases in enhancing crop salt tolerance.
