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Updated: Sep 30, 2025
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Published on: November 16, 2018
Specific microRNAs Regulate Dental Pulp Stem Cell Behavior
Promphakkon Kulthanaamondhita1, Chatvadee Kornsuthisopon1, Suphalak Photichailert1
1Dental Stem Cell Biology Research Unit and Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Introduction:
MicroRNAs (miRNAs), small noncoding RNAs, control the translation of messenger RNAs into proteins. miRNAs have a crucial role in regulating the diverse biological processes of many physiological and pathological activities. The aim of this systematic review was to explore various functions of miRNAs in the regulation of dental pulp stem cell (DPSC) behavior.
Methods:
The articles were searched in PubMed, SCOPUS, and ISI Web of Science database using designated keywords. Full-length manuscripts published in English in peer-reviewed journals relevant to the role of miRNAs in DPSC functions were included and reviewed by 2 independent researchers.
Results:
The original search of the database generated 299 studies. A total of 102 duplicate studies were removed. After their exclusion, 48 studies were selected for review. miRNAs have shown to modulate the stemness and differentiation of various mesenchymal stem cells. The miRNAs expression profiles in DPSCs were differed compared with other cell types and have been demonstrated to regulate the levels of proteins crucial for promoting or inhibiting DPSC proliferation as well as differentiation. Further, miRNAs also modulate inflammatory processes in dental pulp.
Conclusion:
miRNAs have various functions on the regulation of DPSCs and understanding these roles of miRNAs is crucial for the development of new therapeutics in regenerative dental medicine. With the advancing technologies, the utilization of miRNA technology could revolutionarily change the future of regenerative endodontics.
Insights
MicroRNAs (miRNAs) regulate dental pulp stem cell (DPSC) behavior, influencing their stemness, differentiation, and proliferation. Understanding these miRNA functions is key for developing new regenerative dental therapies.
Area of Science:
- Biomedical Science
- Molecular Biology
- Stem Cell Biology
Background:
- MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression.
- miRNAs play critical roles in physiological and pathological processes.
- Dental pulp stem cells (DPSCs) are crucial for dental tissue regeneration.
Purpose of the Study:
- To systematically review the functions of miRNAs in regulating dental pulp stem cell (DPSC) behavior.
- To explore the impact of miRNAs on DPSC stemness, proliferation, and differentiation.
Main Methods:
- Systematic literature search of PubMed, SCOPUS, and ISI Web of Science databases.
- Inclusion of full-length, peer-reviewed English manuscripts.
- Review conducted by two independent researchers.
Main Results:
- miRNAs modulate stemness and differentiation in mesenchymal stem cells, including DPSCs.
- DPSC miRNA expression profiles differ from other cell types.
- miRNAs regulate key proteins affecting DPSC proliferation, differentiation, and inflammatory processes.
Conclusions:
- miRNAs significantly regulate DPSC functions.
- Understanding miRNA roles is vital for novel therapeutics in regenerative dentistry.
- miRNA technology holds revolutionary potential for regenerative endodontics.
