Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Growth Measurement: Indirect Methods

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dental age estimation in the Chhattisgarhi population: A comparative study of Demirjian and Willems methods.

Journal of forensic and legal medicine·2026

Same author

Light-Harvesting O-Doped Graphitic Carbon Nitride Nanosheets Enable Sustainable Algal Growth and Enhanced Metabolite Production in Scenedesmus obliquus.

ACS applied bio materials·2026

Same author

Targeting phospholipase A2 with arachidonyl trifluoromethyl ketone modulates macrophage activation during Japanese encephalitis virus infection.

Medical microbiology and immunology·2026

Same author

FcRn-targeted therapeutics: Neonatal fc receptor at the Interface of balancing immune modulation and persevering protective humoral immunity.

International immunopharmacology·2026

Same author

Developmental origin and clinical profile of siamese twins in a tertiary care setting.

Bioinformation·2026

Same author

Stepped care for young people at clinical high risk for psychosis: a real-world study.

medRxiv : the preprint server for health sciences·2026

Search research articles

Related Experiment Video

Updated: Sep 30, 2025

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Published on: September 20, 2016

Nutrient acclimation in benthic diatoms with adaptive laboratory evolution.

Abhishek Saxena¹, Bharti Mishra¹, Raveendran Sindhu²

¹Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh 201301, India.

Bioresource Technology

|March 10, 2022

Summary

Marine diatoms like Chaetoceros gracilis and Thalassiosira weissflogii were cultivated using agricultural fertilizers and carbon sources. Adaptive laboratory evolution significantly enhanced their growth and biochemical productivity, showing potential for industrial applications.

Keywords:

Adaptive laboratory evolution Agriculture fertilizer Chrysolaminarin Diatom Mixotrophy

More Related Videos

Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution

Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution

Published on: June 14, 2024

Resurrection of Dormant Daphnia magna: Protocol and Applications

Resurrection of Dormant Daphnia magna: Protocol and Applications

Published on: January 19, 2018

Related Experiment Videos

Last Updated: Sep 30, 2025

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Published on: September 20, 2016

Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution

Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution

Published on: June 14, 2024

Resurrection of Dormant Daphnia magna: Protocol and Applications

Resurrection of Dormant Daphnia magna: Protocol and Applications

Published on: January 19, 2018

Area of Science:

Marine biology
Phycology
Biotechnology

Background:

Marine diatoms are crucial primary producers.
Optimizing diatom cultivation is key for sustainable aquaculture and biofuel production.
Adaptive laboratory evolution (ALE) offers a method to enhance microalgal traits.

Purpose of the Study:

To evaluate the growth and productivity of marine diatoms (Chaetoceros gracilis and Thalassiosira weissflogii) under various agricultural fertilizers and carbon sources.
To enhance diatom cell density, biomass, and biochemical content through ALE.
To identify optimal conditions for maximizing diatom growth and valuable compound production.

Main Methods:

Cultivation of C. gracilis and T. weissflogii using different NPK fertilizers and carbon sources (urea, glycerol, sucrose, glucose).
Application of adaptive laboratory evolution (ALE) to acclimate diatoms under optimized conditions.
Measurement of cell density, carbohydrate, protein, lipid, pigment, and chrysolaminarin content.

Main Results:

C. gracilis achieved highest cell density in NPK (202.5 × 10^5 cells mL^-1) and maximum carbohydrate/protein in urea.
T. weissflogii showed highest cell density in glycerol (148.2 × 10^5 cells mL^-1), with maximum carbohydrate in glycerol and protein in sucrose.
Lipid content was highest in glycerol for C. gracilis and glucose for T. weissflogii. Both species exhibited increased pigment and chrysolaminarin production.

Conclusions:

ALE is an effective strategy for enhancing the growth and productivity of marine diatoms.
Specific fertilizers and carbon sources significantly influence diatom growth and biochemical composition.
Optimized cultivation of diatoms holds promise for biotechnological applications, including biofuel and nutraceuticals.