Related Experiment Video
Updated: Sep 30, 2025
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
Published on: February 24, 2021
Novel Protocols for the Production of Autologous Blood Concentrates With High Platelet Volume
Renato Rossi1, Arun K Garg2, Gregori M Kurtzman3
1Director, Oral and Maxillofacial Surgery Residency Program, and Dean, School of Dentistry, University of São Caetano do Sul, São Paulo, Brazil; Private Practice limited to Oral and Maxillofacial Surgery and Oral Pathology, São Paulo, Brazil.
Abstract:
Autologous blood concentrates (ABCs), specifically platelet-rich plasma (PRP), were originally discussed in the literature more than 60 years ago. Since then, protocols to fabricate PRP from patient-derived blood have steadily evolved with the aim of creating better products with more effective clinical results. Key to the protocol evolution has been the development of ABCs with higher concentrates of platelets and other blood components that enhance soft- and hard-tissue healing and, hence, regeneration. The authors present a protocol for obtaining high-platelet-concentration PRP and autogenous thrombin. This fifth-generation protocol allows PRP to be attained with a platelet concentration six times higher than that which is present in the original clot. This double-centrifugation PRP protocol is relatively simple and inexpensive, as it does not require special equipment or advanced technical knowledge by the practitioner and can readily be conducted in a private dental clinical setting.
More Related Videos
12:40Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
Published on: December 7, 2012
08:32Preparation of Pooled Human Platelet Lysate pHPL as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
Published on: October 30, 2009