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Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
Published on: January 21, 2020
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A Mouse Periodontitis Model With Humanized Oral Bacterial Community
Lan Bai1,2,3, Bo-Yan Chen1,2,3, Yan Liu2,3
1Department of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Frontiers in Cellular and Infection Microbiology
|March 11, 2022
Summary
A new mouse model effectively replicates human periodontitis by using patient plaques and ligature placement. This model allows for studying the impact of human oral bacteria on periodontitis and systemic diseases.
Area of Science:
- Microbiology
- Oral Biology
- Immunology
Background:
- Periodontitis, an oral dysbiosis, is linked to systemic disease progression.
- Existing mouse models lack colonization of human periodontitis-associated bacteria.
Purpose of the Study:
- To establish a novel mouse model for periodontitis using human oral microbiota.
- To investigate the colonization and impact of human periodontitis bacteria in mice.
Main Methods:
- Combined ligation of second molars with subgingival plaque application from periodontitis patients.
- 16S rRNA gene sequencing and taxonomic classification for bacterial analysis.
- Assessment of bone resorption and gene expression related to inflammation and osteoclastogenesis.
Main Results:
- Human periodontitis-associated bacteria successfully colonized the mouse model.
- Key pathogens like P. gingivalis and F. nucleatum were enriched.
- The model showed exacerbated periodontitis with increased bone loss and inflammatory gene expression.
Conclusions:
- The new mouse model accurately reflects human periodontitis with human bacterial colonization.
- This model is valuable for studying oral and systemic diseases linked to periodontitis.
