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Updated: Sep 30, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete chloroplast genome sequence of Trigonotis peduncularis (Boraginaceae)
Jian-Hua Wu1, Hui-Min Li2, Jin-Man Lei3
1College of Pharmacy, Pingxiang Health Vocational College, Pingxiang, Jiangxi, China.
Abstract:
Trigonotis peduncularis (Boraginaceae) is an annual or biannual herb widely distributed in temperate Asia and East Europe. The complete chloroplast genome of T. peduncularis was sequenced by high-throughput technologies and assembled for the first time. The complete chloroplast genome of T. peduncularis was 147,508 bp in length with a GC content of 37.6%, which includes a large single-copy region (80,546 bp), a pair of inverted repeats (24,877 bp), and a small single copy (17,208 bp). GC content of IR regions (43.3%) were higher than LSC (35.5%) and SSC (31.1%) regions. The genome was predicted to encode 130 genes, of which 114 were unique, including 80 protein-coding genes, 30 tRNA genes, and four rRNA genes. Result from phylogenetic analysis showed that T. peduncularis was sister to Plagiobothrys nothofulvus, and the intergeneric relationships among the six genera sampled in Boraginaceae were well resolved and strongly supported.
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