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SNHG10/miR-141-3p/WTAP axis promotes osteosarcoma proliferation and migration.

Jiejie Ge1, Meng Liu2, Yuchao Zhang3

  • 1Department of Clinical Laboratory, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, China.

Journal of Biochemical and Molecular Toxicology
|March 11, 2022
PubMed
Summary

The long non-coding RNA SNHG10 promotes osteosarcoma (OS) progression by increasing WTAP levels via sponging miR-141-3p. Inhibiting SNHG10 suppresses OS cell growth and metastasis, offering potential therapeutic targets.

Keywords:
OSWTAPlncRNA SNHG10miR-141-3pprogression

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Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in cancer development and metastasis.
  • SNHG10, a recently identified lncRNA, shows oncogenic potential in osteosarcoma (OS).
  • The precise molecular mechanisms of SNHG10 in OS remain largely unelucidated.

Purpose of the Study:

  • To investigate the role and mechanism of SNHG10 in osteosarcoma (OS) progression.
  • To elucidate the regulatory axis involving SNHG10, miR-141-3p, and WTAP in OS.

Main Methods:

  • Quantitative real-time PCR to assess gene expression levels.
  • Cell proliferation, migration, invasion, and apoptosis assays.
  • RNA immunoprecipitation (RIP) and dual-luciferase reporter assays to confirm molecular interactions.

Main Results:

  • SNHG10 expression was significantly upregulated in OS tissues and cells.
  • Knockdown of SNHG10 inhibited OS cell proliferation, migration, and invasion, while promoting apoptosis.
  • SNHG10 directly targeted miR-141-3p, leading to increased WTAP expression, thereby promoting OS progression.

Conclusions:

  • The SNHG10/miR-141-3p/WTAP axis plays a crucial role in facilitating osteosarcoma progression.
  • SNHG10 acts as an oncogene in OS by modulating the miR-141-3p/WTAP pathway.
  • Targeting the SNHG10/miR-141-3p/WTAP axis may represent a novel therapeutic strategy for osteosarcoma.