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Assessment of in vitro growth characteristics of Mycoplasma ovipneumoniae
Abstract:
Mycoplasma ovipneumoniae (Movp) is an emerging pathogen that causes respiratory disease in small ruminants worldwide. It is considered to be difficult and time consuming to grow, which complicates diagnostic and control measures including isolation (an essential step required prior to the characterisation of strains), antimicrobial susceptibility testing and the development of vaccines. The objectives of this study were to analyse in vitro growth patterns of Movp strains, and the effects of different media used to support their growth. The study was conducted on 20 ovine and caprine Movp strains, isolated using Thiaucourt's medium. The rapid growth phase varied among the strains from 24 h to 72 h, although 60% of strains (12 of 20) reached a peak at 48 h. All strains were viable at 72 h after incubation, and declining viability was observed at 96 h (13 of 20 remained viable; 65%), 120 h (9 of 20; 45%) and 144 h (4 of 20; 20%). Growth was not detected at 168 h. All strains were able to grow in modified tryptone soy broth, while PH mycoplasma medium-Hayflick modified medium supported the growth of only two strains. Improved techniques of Movp cultivation require consideration of the growth variability among strains, the time of subculturing (during the first three days of incubation) and selection of appropriate media.
Insights
Mycoplasma ovipneumoniae (Movp) growth varies significantly between strains. Optimizing cultivation requires careful media selection and subculturing within the first three days for effective diagnostics and control of this ovine and caprine pathogen.
Area of Science:
- Veterinary Microbiology
- Animal Pathology
Background:
- Mycoplasma ovipneumoniae (Movp) is a significant emerging pathogen causing respiratory illness in small ruminants globally.
- Difficult and time-consuming cultivation of Movp hampers diagnostics, antimicrobial susceptibility testing, and vaccine development.
Purpose of the Study:
- To analyze in vitro growth patterns of Mycoplasma ovipneumoniae strains.
- To evaluate the efficacy of different culture media for Mycoplasma ovipneumoniae growth.
Main Methods:
- Twenty ovine and caprine Movp strains were isolated using Thiaucourt's medium.
- In vitro growth patterns and viability were monitored over 168 hours.
- Growth was assessed across modified tryptone soy broth and PH mycoplasma medium-Hayflick modified medium.
Main Results:
- The rapid growth phase for Movp strains ranged from 24 to 72 hours, with 60% peaking at 48 hours.
- All strains remained viable at 72 hours, with declining viability observed from 96 to 144 hours.
- Modified tryptone soy broth supported growth of all strains, while PH mycoplasma medium-Hayflick supported only two.
Conclusions:
- Movp cultivation success depends on strain-specific growth variability and appropriate media selection.
- Subculturing within the first three days of incubation is crucial for Mycoplasma ovipneumoniae.
- Optimized cultivation techniques are essential for advancing Movp diagnostics and control strategies.
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