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Updated: Sep 30, 2025

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
Published on: December 11, 2020
The Melatonin Receptor CAND2/PMTR1 Is Involved in the Regulation of Mitochondrial Gene Expression under
I A Bychkov1, N V Kudryakova2, A G Shugaev2
1Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia. Ivan.a.b@mail.ru.
Abstract:
Melatonin is a signaling molecule that mediates multiple stress-dependent reactions. Under photooxidative stress conditions generating intensive ROS production, exogenous melatonin (50 µM) contributed to maintaining the expression of mitochondrial encoded genes and up-regulation of RNA-polymerase genes RPOTm and RPOTmp, operating through the CAND2 receptor and α-subunit of the heterotrimeric G protein GPA1 coupled with CAND2. Unlike wild-type plants, mutants with defective CAND2 and GPA1 genes exhibited no decrease in the alternative pathway of leaf respiration, as well as the activity of an alternative oxidase, and the expression of the AOX1a gene. At the same time, the protective effect of exogenous melatonin on some physiological indicators did not depend on the receptor and was associated with the direct antioxidant function of the regulator. Thus, melatonin under photooxidative stress conditions can act as an antioxidant and as a hormone capable of regulating the expression of nuclear and organelle genes through the components of melatonin signal perception.
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