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Related Experiment Videos

Simple solution to problem of biostator-induced insulin aggregation.

M D Harris, M B Davidson, C S Rosenberg

    Diabetes Care
    |July 1, 1986
    PubMed
    Summary

    Continuous insulin infusion using the Biostator system can cause insulin loss. Adding heparinized whole blood to the insulin reservoir prevents heat-induced insulin aggregation, ensuring accurate study data.

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    Area of Science:

    • Biomedical Engineering
    • Endocrinology
    • Clinical Research

    Background:

    • Prolonged insulin delivery via the Biostator system leads to heat-induced insulin aggregation.
    • This aggregation significantly reduces insulin immunoreactivity and bioavailability.
    • Consequently, data from studies using continuous Biostator insulin infusions become uninterpretable.

    Purpose of the Study:

    • To identify and address the issue of insulin loss during Biostator-assisted continuous insulin delivery.
    • To propose a simple and effective method to prevent heat-induced insulin aggregation.

    Main Methods:

    • Investigated the artifact of insulin aggregation in the Biostator insulin delivery system.
    • Proposed adding 20 ml of heparinized whole blood to the 500-ml insulin reservoir.

    Main Results:

    • The addition of heparinized whole blood effectively prevented insulin aggregation.
    • This method maintained insulin immunoreactivity and bioavailability during prolonged infusion.
    • The solution was found to be simple, economical, and safe for subjects.

    Conclusions:

    • Heparinized whole blood addition is a viable solution to prevent insulin loss in Biostator systems.
    • This technique ensures the integrity of data from studies employing continuous insulin infusions.
    • The method offers a practical and risk-free approach for researchers.

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