Related Experiment Video
Updated: Sep 30, 2025
Studying Orthodontic Tooth Movement in Mice
Published on: August 2, 2024
One-stage versus two-stage piezocision-assisted orthodontic tooth movement: A preclinical study based on Nano-CT and
Carole Charavet1, Dorien Van Hede2, Sandy Anania3
1Université Côte d'Azur, Faculté de Chirurgie-Dentaire, Département d'Orthodontie, Nice, France; CHU de Nice, Pôle Odontologie, UEC d'Orthodontie, Nice, France; Laboratoire Microbiologie orAle Immunité Santé MICORALIS (UPR 7354), Université Côted'Azur, France; Dental Biomaterials Research Unit, University of Liège, Belgium.
Objective:
To evaluate the effect of a second-stage piezocision on the biological response.
Materials And Methods:
60 rats were randomly allocated to 6 experimental groups of 10 rats. Rats undergoing a one-stage piezocision were sacrified on day 7, 28 and 42 (groups 1-3) while rats undergoing a two-satge piezocision were sacrified on day 42, 63 and 90 (groups 4-6), respectively. The biological response was investigated in 3D at the tissue level using Nano-computed tomography (Nano-CT) and, at the molecular level using the qRT-PCR technique. Bone Volume Fraction (BVF) loss was the primary endpoint.
Results:
Similar loss of BVF were observed both after the first and second piezocisions. The change in BVF loss between 7 and 28 days after each piezocision were 25.1 ± 13.0 (SE)% and 11.2 ± 11.6 (SE)% respectively and did not differ from each other (p = 0.43). Changes in BVF loss from 7 to 42 days were also comparable in one-stage and two-stage piezocision (4.9 ± 12.3 (SE) vs. -19.9 ± 13.4 (SE), p = 0.19). At the molecular level, all parameters except Translating Ribosome Affinity Purification (TRAP) protein had identical patterns.
Conclusion:
Within the limits of the present study, a second piezocision allowed to re-induce the Regional Acceleratory Phenomenon (RAP) effect. Nevertheless, the relevance of the findings to the clinical effect has not been tested.
