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Updated: Sep 30, 2025

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Industrial production of microbial protein products
Mason Banks1, Rob Johnson2, Lori Giver3
1Department of Engineering, Faculty of Natural Mathematical & Engineering Sciences, King's College London, Strand Campus, WC2R 2LS, UK.
Abstract:
Microbial proteins provide a sustainable and nutritious alternative to traditional animal and plant-based proteins. Various strains have been demonstrated to generate biomass from a wide variety of substrates, from organic waste (e.g. banana peel) to gases (e.g. methane). Industrial production of microbial protein has proven difficult from both design (e.g. production rate) and regulatory (e.g. allergenicity of product) perspectives for both feed and food-grade products. Through use of low-cost microfluidics devices, early bioprocess design can be intensified, achieving high strain screening throughput with low titres. Integration of industrial waste streams (e.g. flue-gases, lignocellulosic residues) can reduce cost and carbon footprint of feedstock, while bespoke reactor design (e.g. Quorn's airlift U-loop fermenter) can remediate issues of low mass-transfer and product quality.
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