Related Experiment Video
Updated: Sep 30, 2025
12:29
mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
9.3K
Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing
Li Fan1, Katja Fröhlich1, Eric Melzer1,2
1Center of Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany.
Nature Communications
|March 12, 2022
Summary
Plant pattern-triggered immunity (PTI) is activated by recognizing microbial patterns. Researchers identified translation initiation factor 1 (IF1) as a pattern that triggers PTI, with RLP32 identified as its specific receptor.
Area of Science:
- Plant immunology
- Molecular biology
- Genetics
Background:
- Plant pattern-triggered immunity (PTI) involves pattern recognition receptors (PRRs) detecting microbial patterns.
- The specific receptors for many bacterial elicitors, like translation initiation factor 1 (IF1), remain largely unknown.
Purpose of the Study:
- To identify the plant receptor for the proteobacterial pattern IF1.
- To elucidate the mechanism of IF1 recognition and downstream signaling in plant immunity.
Main Methods:
- Utilized natural variation in IF1 sensitivity within Arabidopsis accessions.
- Employed restriction site-associated DNA sequencing (RAD-seq) for genetic mapping.
- Performed binding assays and complex formation studies with known signaling components.
Main Results:
- Identified Leucine-Rich Repeat Receptor-Like Protein 32 (RLP32) as the IF1 receptor in Arabidopsis thaliana.
- Demonstrated that RLP32 specifically binds IF1 and mediates PTI signaling through SOBIR1 and BAK1.
- Showcased RLP32's role in conferring resistance against Pseudomonas syringae.
Conclusions:
- RLP32 is a novel PRR that recognizes the proteobacterial IF1 pattern, requiring its tertiary structure.
- Plant immune systems possess a complex and diverse repertoire of pattern sensors, even within a single species.
More Related Videos
Related Concept Videos
01:25Cell Signaling in Plants
5.8K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.8K
02:24Ribosome Profiling
3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K
02:28Leaky Scanning
5.3K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.3K
01:56Riboswitches
8.7K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.7K
01:34Directing Proteins to the Rough Endoplasmic Reticulum
10.0K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
10.0K