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Related Experiment Video

Updated: Sep 30, 2025

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

Comprehensive 3D epigenomic maps define limbal stem/progenitor cell function and identity.

Mingsen Li¹, Huaxing Huang¹, Bofeng Wang¹

¹State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, China.

Nature Communications

|March 12, 2022

Summary

This summary is machine-generated.

This study maps human limbal stem cell (LSC) genome topology, revealing how 3D genome organization controls gene expression and cell identity. Findings illuminate epigenetic regulation in stratified epithelia.

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Last Updated: Sep 30, 2025

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Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

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Published on: December 18, 2015

Area of Science:

Genomics
Epigenetics
Stem Cell Biology

Background:

Understanding genome topology's role in corneal epithelium function is limited.
The interplay between 3D genome organization and epigenetic states is crucial for cell identity.

Purpose of the Study:

To investigate how genome topology and epigenetic states govern corneal epithelium function.
To characterize the 3D epigenomic landscapes of human limbal stem/progenitor cells (LSCs).

Main Methods:

High-resolution Hi-C interaction mapping of human LSCs.
Integration of Hi-C, epigenome, and transcriptome data.
Analysis of super-enhancer (SE) interactions and chromatin looping.

Main Results:

Chromatin multi-hierarchical organization is coupled to gene expression in LSCs.
Super-silencers mediate gene repression, while SEs drive LSC-specific gene activation.
Cohesin-occupied CTCF-CTCF loops anchor regulatory networks.

Conclusions:

Detailed insights into genome organization principles for epigenetic regulation in stratified epithelia.
Identification of regulatory networks involving transcription factors and SE-promoter interactions.
Elucidation of mechanisms governing LSC function and identity through 3D genome architecture.