Related Experiment Video

Updated: Sep 30, 2025

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation
11:07

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Published on: July 6, 2019

25.6K

Long noncoding RNA MIR3142HG accelerates lipopolysaccharide-induced acute lung injury via miR-95-5p/JAK2 axis

Yanqiu Gao1, Shuangfeng Li2, Rui Dong2

  • 1Respiratory Intensive Care Unit (RICU), Zhengzhou Central Hospital Affiliated to Zhengzhou University, 16 Tongbai North Road, Zhengzhou, 450007, People's Republic of China. hiuk133@163.com.

Human Cell
|March 12, 2022
PubMed

Insights

MIR3142HG is upregulated in acute lung injury (ALI). This long non-coding RNA accelerates ALI progression by targeting the miR-95-5p/JAK2 pathway, suggesting MIR3142HG as a potential therapeutic target for ALI.

Area of Science:

  • Molecular Biology
  • Immunology
  • Pulmonary Medicine

Background:

  • Acute lung injury (ALI) is a critical condition with limited therapeutic options.
  • The role of long non-coding RNAs (lncRNAs) like MIR3142HG in ALI pathogenesis is not fully understood.
  • The JAK/STAT signaling pathway is implicated in inflammatory responses during ALI.

Purpose of the Study:

  • To elucidate the expression profile of MIR3142HG in acute lung injury (ALI).
  • To investigate the mechanistic role of MIR3142HG in regulating the JAK/STAT pathway in ALI.
  • To explore MIR3142HG as a potential therapeutic target for ALI.

Main Methods:

  • Analysis of MIR3142HG and JAK2 expression in serum samples from ALI patients.
  • In vitro experiments using lipopolysaccharide (LPS)-induced ALI cell models.
  • Knockdown and overexpression of MIR3142HG and miR-95-5p.
  • Rescue experiments to validate molecular interactions.

Main Results:

  • MIR3142HG and JAK2 were significantly overexpressed in ALI patient serum.
  • MIR3142HG knockdown enhanced cell viability and suppressed apoptosis and inflammation in LPS-treated ALI cells via miR-95-5p.
  • Overexpression of miR-95-5p promoted cell viability and inhibited apoptosis/inflammation by targeting JAK2 in LPS-induced ALI cells.
  • Inhibition of miR-95-5p reversed the effects of MIR3142HG knockdown.

Conclusions:

  • MIR3142HG is upregulated in sepsis-induced ALI.
  • MIR3142HG exacerbates ALI progression through the miR-95-5p/JAK2 axis.
  • MIR3142HG represents a promising therapeutic target for acute lung injury.
Keywords:
Acute lung injuryJAK2MIR3142HGmiR-95-5p

More Related Videos

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury08:27

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Published on: May 21, 2018

11.1K
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury14:48

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Published on: March 21, 2021

5.2K

Related Concept Videos

The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.5K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K