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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
Published on: August 12, 2019
Transcriptome Analysis Reveals that MAPK Signaling Pathway Mediates Salt Tolerance of YMR253C ORF in Saccharomyces
Yu Zhang1,2, Mengyan Li2, Juqing Deng2
1School of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming, 650500, Yunnan, China.
Abstract:
The YMR253C open reading frame encodes a membrane protein that is highly expressed in NaCl-resistant Saccharomyces cerevisiae mutants. Whether it mediates NaCl tolerance is unclear. By knocking out YMR253C in S. cerevisiae, we found that the salt tolerance of yeast was reduced, the integrity of the cell wall was impaired, and cell death was induced; transcriptome analysis further revealed that YMR253C gene knockout mediates significant changes of 1291 genes, and YMR253C mediates the regulation of MAPK signal pathways. Therefore, the transmembrane protein YMR253C may regulate the MAPK signaling pathway to regulate the salt stress of S. cerevisiae.
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