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New insights into the DNA repair pathway choice with NuA4/TIP60

Anahita Lashgari1, Pata-Eting Kougnassoukou Tchara2, Jean-Philippe Lambert2

  • 1St-Patrick Research Group in Basic Oncology, Canada; Laval University Cancer Research Center, CHU de Québec-Université Laval Research Center, Quebec City, QC, Canada; Department of Molecular Medicine, Big Data Research Center, Université Laval, Quebec, Canada.

DNA Repair
|March 12, 2022
PubMed

Insights

The NuA4/TIP60 complex influences DNA double-strand break (DSB) repair pathway choice by regulating chromatin dynamics and modifying proteins. This chromatin remodeling is crucial for homologous recombination and DNA repair in eukaryotic cells.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Eukaryotic cells repair DNA double-strand breaks (DSBs) via non-homologous end-joining (NHEJ) or homologous recombination (HR).
  • Pathway selection depends on antagonistic repair factors and cell cycle status.
  • Chromatin modifications around DSBs are key to this decision.

Purpose of the Study:

  • To review recent advances in understanding the NuA4/TIP60 complex's role in DSB repair.
  • To highlight NuA4/TIP60's contribution to DNA repair pathway choice.
  • To elucidate how NuA4/TIP60 regulates chromatin dynamics and DNA end resection.

Main Methods:

  • Literature review of recent advances in DNA repair research.
  • Focus on the function of the NuA4/TIP60 histone acetyltransferase/chromatin remodeling complex.
  • Discussion of the interplay between NuA4/TIP60, SAGA complex, and DNA repair pathways.

Main Results:

  • NuA4/TIP60 plays a critical role in selecting the DNA repair pathway.
  • This complex collaborates with the SAGA complex to modulate chromatin.
  • NuA4/TIP60 modifies non-histone substrates, facilitating DNA end resection and recombination.

Conclusions:

  • NuA4/TIP60 is a key regulator of DNA double-strand break repair pathway choice.
  • Chromatin remodeling by NuA4/TIP60 is essential for homologous recombination.
  • Understanding NuA4/TIP60 function provides insights into maintaining genomic stability.
Keywords:
Chromatin remodelingDouble-strand breakHistone modificationsNuA4SAGATIP60

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