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Related Experiment Video

Updated: Sep 30, 2025

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Genome-wide investigation of maize RAD51 binding affinity through phage display.

Claire Milsted1,2, Bo Dai2, Nelson Garcia2,3

  • 1School of Life Sciences, Arizona State University, 427 E Tyler Mall, Tempe, AZ, 85287, USA.

BMC Genomics
|March 13, 2022
PubMed
Summary

This study identified new maize RAD51A1 protein interactions using phage display, revealing potential roles in plant defense and transcriptional regulation. These findings advance our understanding of DNA repair and plant immunity.

Keywords:
BRCA2MaizePeptidePhage DisplayRAD51Transcription factorZea mays

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Area of Science:

  • Plant molecular biology
  • Genetics
  • Biochemistry

Background:

  • RAD51 proteins are crucial for DNA repair and homologous recombination in eukaryotes.
  • While RAD51's role in plant defense is suggested in Arabidopsis, its function and interactors in maize remain largely unexplored.
  • Identifying RAD51 interactors can elucidate meiotic recombination and potential defense mechanisms in maize.

Purpose of the Study:

  • To identify protein-protein interactions with maize RAD51A1 (ZmRAD51A1).
  • To investigate potential roles of ZmRAD51A1 in plant defense and transcriptional regulation.
  • To demonstrate the utility of phage display for discovering protein interactors in basic science.

Main Methods:

  • Phage display screening of a random peptide library against maize RAD51A1.
  • In vitro validation of phage-protein interactions using ELISA and dot blotting.
  • Synthesis and testing of custom peptides based on maize proteome sequences.

Main Results:

  • Eleven peptide sequences from phages bound ZmRAD51A1 in vitro; nine interactions were confirmed.
  • One phage peptide showed significant alignment to a maize BRCA2 binding domain.
  • Fourteen synthesized peptides, derived from maize proteins including transcription factors, bound ZmRAD51A1.

Conclusions:

  • Several peptides interacting with ZmRAD51A1 were identified.
  • Results suggest ZmRAD51A1 may be involved in transcriptional regulation and plant defense.
  • Phage display is a viable method for identifying protein binding partners in maize.