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Circ_0001174 facilitates osteosarcoma cell proliferation, migration, and invasion by targeting the miR-186-5p/MACC1

Feifei Lin1, Xiaonan Wang1, Xin Zhao1

  • 1Department of Orthopedics, The Second Hospital of Jilin University, Ziqiang Street 218, Changchun, 130041, Jilin, China.

Journal of Orthopaedic Surgery and Research
|March 13, 2022
PubMed
Abstract

Insights

Circular RNA circ_0001174 promotes osteosarcoma (OS) progression by increasing MACC1 via sponging miR-186-5p. Targeting circ_0001174 offers a potential therapeutic strategy for osteosarcoma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrantly expressed circular RNAs (circRNAs) are implicated in osteosarcoma (OS) pathogenesis.
  • The specific role of circ_0001174 in OS progression was previously unknown.

Purpose of the Study:

  • To identify differentially expressed circRNAs and messenger RNAs (mRNAs) in OS tissues.
  • To investigate the regulatory mechanisms of circ_0001174 in OS.

Main Methods:

  • High-throughput sequencing and RT-qPCR for differential expression analysis.
  • In vitro assays (CCK-8, migration, invasion) to assess circRNA function.
  • RNA interference and Western blotting to elucidate molecular interactions (circ_0001174, miR-186-5p, MACC1).

Main Results:

  • 109 circRNAs and 1264 mRNAs were differentially expressed in OS tissues.
  • circ_0001174 was significantly upregulated in OS tissues and cell lines.
  • Knockdown of circ_0001174 suppressed OS cell proliferation, migration, and invasion.
  • circ_0001174 negatively regulated miR-186-5p and positively regulated MACC1.

Conclusions:

  • Upregulated circ_0001174 promotes OS cell proliferation, migration, and invasion.
  • This promotion occurs via the upregulation of MACC1 by sponging miR-186-5p.
  • circ_0001174 represents a potential therapeutic target for osteosarcoma.
Keywords:
Differentially expressed genesMACC1OsteosarcomacircRNAmiR-186-5p

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