Related Experiment Video
Updated: Sep 30, 2025
Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
Long Non-Coding RNA HCG11 Inhibits Glioma Cells Proliferation and Migration through Decoying miR-590-3p and
Ma Jinyang1, Lang Bojuan2, Xue Lixin3
1Department of Neurology, The First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, Yichang, China.
Background:
Long non-coding RNAs are reportedly endowed with the function of promoting or inhibiting cancer occurrence and development. The emphasis of this study was placed on the effect of lncRNA HLA complex group 11 (HCG11) on glioma progression, as well as its mechanism.
Methods:
Quantitative real-time polymerase chain reaction was utilized for detecting HCG11, miR-590-3p, and CAMD2 mRNA expression levels in glioma tissues. Western blot was adopted to examine cell adhesion molecule (CADM2) protein expression. Cell counting kit-8, BrdU, Transwell and wound healing assays were employed for investigating the malignant biological behaviors of glioma cells. RNA immunoprecipitation assay and dual-luciferase reporter assay were performed to prove the relationship between miR-590-3p and HCG11, as well as CADM2 and miR-590-3p.
Results:
HCG11 expression was lower in glioma tissues compared with that in paracancerous tissues, and its expression level was negatively correlated with WHO tumor stage. In addition, compared with in astrocyte cell line, the expression of HCG11 was lower in glioma cells. Functional experiments showed that HCG11 inhibited glioma cells migration and proliferation, while miR-590-3p facilitated these processes. Acting as a competitive endogenous RNA, HCG11 adsorbed miR-590-3p and upregulated the expression of CADM2, the target gene of miR-590-3p.
Conclusions:
HCG11 suppresses glioma cells proliferation and migration through regulating the miR-590-3p/CADM2 molecular axis.
Insights
Long non-coding RNA HLA complex group 11 (HCG11) suppresses glioma progression. HCG11 inhibits cancer cell migration and proliferation by regulating the miR-590-3p/CADM2 axis.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) play critical roles in cancer development.
- The specific role of lncRNA HLA complex group 11 (HCG11) in glioma remains to be fully elucidated.
Purpose of the Study:
- To investigate the effect of HCG11 on glioma progression.
- To explore the underlying molecular mechanism of HCG11 in glioma.
Main Methods:
- Quantitative real-time PCR and Western blot were used to detect gene and protein expression.
- Cell proliferation, migration, and invasion were assessed using CCK-8, BrdU, Transwell, and wound healing assays.
- RNA immunoprecipitation and dual-luciferase reporter assays confirmed molecular interactions.
Main Results:
- HCG11 expression was significantly downregulated in glioma tissues and cells, correlating negatively with WHO tumor stage.
- HCG11 overexpression suppressed glioma cell proliferation and migration, while miR-590-3p promoted these processes.
- HCG11 acted as a competing endogenous RNA, sponging miR-590-3p and consequently upregulating its target gene, CADM2.
Conclusions:
- HCG11 functions as a tumor suppressor in glioma.
- HCG11 inhibits glioma cell proliferation and migration by modulating the miR-590-3p/CADM2 molecular axis.
Related Concept Videos
01:22MicroRNAs
02:39lncRNA - Long Non-coding RNAs
02:15Experimental RNAi
02:23Abnormal Proliferation