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Efficacy of EPA-registered disinfectants against two human norovirus surrogates and Clostridioides difficile
Jinge Huang1, Geun Woo Park2, Rachael M Jones3
1Department of Food, Nutrition, and Packaging Sciences, Clemson University, Clemson, South Carolina, USA.
Aims:
To determine the efficacy of a panel of nine EPA-registered disinfectants against two human norovirus (HuNoV) surrogates (feline calicivirus [FCV] and Tulane virus [TuV]) and Clostridioides difficile endospores.
Methods And Results:
Nine EPA-registered products, five of which contained H2 O2 as active ingredient, were tested against infectious FCV, TuV and C. difficile endospores using two ASTM methods, a suspension and carrier test. Efficacy claims against FCV were confirmed for 8 of 9 products. The most efficacious product containing H2 O2 as ingredient achieved a >5.1 log reduction of FCV and >3.1 log reduction of TuV after 5 min, and >6.0 log reduction of C. difficile endospores after 10 min. Of the five products containing H2 O2 , no strong correlation (R2 = 0.25, p = 0.03) was observed between disinfection efficacy and H2 O2 concentration. Addition of 0.025% ferrous sulphate to 1% H2 O2 solution improved efficacy against FCV, TuV and C. difficile.
Conclusion:
Disinfectants containing H2 O2 are the most efficacious disinfection products against FCV, TuV and C. difficile endospores. Product formulation, rather than the concentration of H2 O2 in a product, impacts the efficacy of a disinfection product.
Significance And Impact Of Study:
H2 O2 -based disinfectants are efficacious against surrogate viruses for HuNoV and C. difficile endospores.
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