Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Concurrent Calcium Pyrophosphate Deposition Disease and Gout of the Cervical Spine Presenting With Subacute Neck Pain and Radiculopathy.

The Journal of rheumatology·2026
Same author

Early prediction of unplanned critical care transfers in children using EHR-based ensemble machine learning.

JAMIA open·2026
Same author

Poplar: A polarized neutron development beamline for polarization analysis and Larmor labeling techniques.

Structural dynamics (Melville, N.Y.)·2026
Same author

Science communication tools: rubrics for generating posters and manuscripts that are authentic to the practice of science.

Journal of microbiology & biology education·2026
Same author

A systematic overexpression screen identifies cytotoxic genes encoded by the Cluster L1 mycobacteriophage LeBron.

G3 (Bethesda, Md.)·2026
Same author

Mitochondrial proteomics reveals reductive metabolism dependent on glutamine in fibroblasts of idiopathic pulmonary fibrosis under hypoxia.

Scientific reports·2026
Same journal

Investigating the antimicrobial efficacy of two aerosolised hydrogen peroxide fumigation cycles for biological safety cabinet decontamination.

Journal of applied microbiology·2026
Same journal

Phytochemical profile and anticryptococcal activity of a phenolic-rich fraction from the bark of Myracrodruon urundeuva M. Allemão.

Journal of applied microbiology·2026
Same journal

Streptococcus thermophilus 1131 and Lactobacillus delbrueckii subsp. bulgaricus 2038 induce immune responses via M cell-mediated transcytosis in an in vitro human gut model.

Journal of applied microbiology·2026
Same journal

The oral phageome in human health, disease, and clinical implications.

Journal of applied microbiology·2026
Same journal

Lactiplantibacillus plantarum LSC3 attenuates anaphylactic response in a murine model of food allergy.

Journal of applied microbiology·2026
Same journal

Isolation, Genomic Analysis, and Evaluation of the Novel Lytic Phage vB_Cf_HW01: Potent Antibiofilm Activity and Therapeutic Efficacy Against a Clinical Isolate of Citrobacter freundii.

Journal of applied microbiology·2026
See all related articles

Related Experiment Video

Updated: Sep 30, 2025

Author Spotlight: Investigating Bacteriophage-Induced Immune Responses in Gnotobiotic Mice
08:46

Author Spotlight: Investigating Bacteriophage-Induced Immune Responses in Gnotobiotic Mice

Published on: January 26, 2024

1.9K

Assessing cross-laboratory performance for quantifying coliphage using EPA Method 1642.

Amity G Zimmer-Faust1, John F Griffith1, Joshua A Steele1

  • 1Southern California Coastal Water Research Project Authority, Costa Mesa, California, USA.

Journal of Applied Microbiology
|March 13, 2022
PubMed
Summary
This summary is machine-generated.

Six laboratories demonstrated consistent performance using the U.S. Environmental Protection Agency (USEPA) Method 1642 for coliphage enumeration in recreational water. This indicates coliphage can be reliably used as a new indicator for beach water quality.

Keywords:
bacteriophageenvironmental/recreational waterindicatorswastewaterwater quality

More Related Videos

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

9.9K
Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography
06:30

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

6.1K

Related Experiment Videos

Last Updated: Sep 30, 2025

Author Spotlight: Investigating Bacteriophage-Induced Immune Responses in Gnotobiotic Mice
08:46

Author Spotlight: Investigating Bacteriophage-Induced Immune Responses in Gnotobiotic Mice

Published on: January 26, 2024

1.9K
A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

9.9K
Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography
06:30

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

6.1K

Area of Science:

  • Environmental microbiology
  • Water quality assessment
  • Public health

Background:

  • Recreational water quality is crucial for public health.
  • Current bacterial indicators like enterococci have limitations.
  • Coliphage are proposed as a more reliable indicator of fecal contamination.

Purpose of the Study:

  • To evaluate the inter-laboratory consistency of the U.S. Environmental Protection Agency (USEPA) Method 1642 for coliphage enumeration.
  • To determine if laboratories can meet performance goals for this new method.
  • To assess the suitability of coliphage as a recreational water quality indicator.

Main Methods:

  • Six laboratories participated in a blind study.
  • Samples included wastewater-spiked, coliphage-spiked, and laboratory blanks.
  • Performance was assessed based on recovery requirements and failure rates for somatic and F+ coliphage.

Main Results:

  • All laboratories met average recovery requirements across samples.
  • Initial failures by less-experienced labs were correctable.
  • Somatic coliphage showed higher failure rates due to stringent criteria.
  • No significant difference in failure rates between marine water and phosphate-buffered saline matrices.

Conclusions:

  • Laboratory performance is adequate for widespread adoption of USEPA Method 1642.
  • Coliphage show comparable inter-laboratory variation to enterococci.
  • Coliphage are a viable alternative indicator for recreational water health risk assessment.