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CircATRNL1 and circZNF608 Inhibit Ovarian Cancer by Sequestering miR-152-5p and Encoding Protein

Mengmeng Lyu1, Xiujuan Li2, Yang Shen1

  • 1Department of Gynecologic Oncology, The Affiliated Cancer Hospital of Nanjing Medical University and Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research, Nanjing, China.

Frontiers in Genetics
|March 14, 2022
PubMed

Insights

Two circular RNAs, circATRNL1 and circZNF608, are downregulated in ovarian cancer and act as tumor suppressors. CircZNF608 inhibits cancer by binding miR-152-5p, while circATRNL1 may encode a protein.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in various disease pathologies.
  • Their specific roles in ovarian cancer pathogenesis require further elucidation.

Purpose of the Study:

  • To investigate the expression and function of circRNAs in ovarian cancer.
  • To identify specific circRNAs involved in ovarian cancer development and progression.

Main Methods:

  • RNA sequencing to analyze circRNA expression in ovarian cancer tissues versus normal tissues.
  • Fluorescent in situ hybridization (FISH), proliferation, and Transwell assays to assess circRNA function.
  • Luciferase reporter assays to validate miRNA-circRNA interactions.

Main Results:

  • CircATRNL1 and circZNF608 were found to be significantly downregulated in ovarian cancer tissues.
  • Overexpression of circATRNL1 and circZNF608 suppressed ovarian cancer cell proliferation and invasion.
  • CircZNF608 directly interacts with miR-152-5p, and circATRNL1 potentially encodes a 131 amino acid protein.

Conclusions:

  • CircATRNL1 and circZNF608 function as tumor suppressors in ovarian cancer.
  • CircZNF608's antitumor effects may be mediated through its interaction with miR-152-5p.
  • CircATRNL1's potential to encode a protein warrants further investigation into its functional mechanisms.
Keywords:
circular RNAovarian cancerpeptideproteintranslation

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