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Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

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Related Experiment Video

Updated: Sep 30, 2025

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Published on: October 31, 2012

CD73⁺ adipose-derived stem cells reduce scar formation through PLOD1.

Miao Xu¹, Shuo Fang², Xiaorong Ma¹

¹Department of Plastic and Reconstructive Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Annals of Translational Medicine

|March 14, 2022

Summary

Adipose-derived mesenchymal stem cells (AMSCs) expressing CD73 show improved wound healing by reducing fibrosis. This occurs through decreased procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1) levels, suggesting CD73+ AMSCs are more effective for scar reduction.

Keywords:

CD73 Scar formation adipose-derived mesenchymal stem cells (AMSCs)procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1)

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Last Updated: Sep 30, 2025

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

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Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Published on: August 4, 2017

Area of Science:

Regenerative Medicine
Stem Cell Therapy
Dermatology

Background:

Cutaneous scar formation is a key factor in assessing skin wound healing success.
Adipose-derived mesenchymal stem cells (AMSCs) show therapeutic potential for wound healing.
Suppression of procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1) in AMSCs reduces scarring, potentially via macrophage polarization modulation.

Purpose of the Study:

To investigate if a CD73-positive (CD73+) subpopulation of AMSCs reduces scar formation more effectively than CD73-negative (CD73-) AMSCs.
To explore the role of PLOD1 levels in CD73+ versus CD73- AMSCs concerning scar reduction.

Main Methods:

Gene profiling of CD73+ and CD73- AMSCs from a public database (GSE167219).
Isolation and flow cytometry sorting of CD73+ and CD73- AMSCs from mouse adipose tissue.
Manipulation of PLOD1 levels in AMSCs (depletion via sh-PLOD1 or overexpression via transgene) and transplantation into induced skin injuries in mice to assess fibrosis.

Main Results:

CD73+ AMSCs exhibited significantly lower PLOD1 levels compared to CD73- AMSCs.
Transplantation of CD73+ AMSCs resulted in reduced skin injury site fibrosis compared to CD73- AMSCs.
Restoring PLOD1 in CD73+ AMSCs negated the anti-fibrosis effect, while PLOD1 depletion in CD73- AMSCs improved fibrosis outcomes.

Conclusions:

CD73+ AMSCs represent a subpopulation with enhanced therapeutic efficacy in wound healing.
These cells inhibit scar formation indirectly through reduced PLOD1 expression.
The findings highlight the potential of targeting CD73+ AMSCs for improved scar management.