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PCR

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The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jun 20, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

T-Cup: A Cheap, Rapid, and Simple Home Device for Isothermal Nucleic Acid Amplification.

Aldrik H Velders¹, Michel Ossendrijver², Bart J F Keijser²

¹Laboratory of BioNanoTechnology Wageningen University P.O. Box 8038 Wageningen 6700EK The Netherlands.

Global Challenges (Hoboken, NJ)

|March 14, 2022

Summary

A new, low-cost, eco-friendly device enables at-home SARS-CoV-2 RNA detection using loop-mediated amplification (LAMP) tests. This decentralized approach bypasses centralized testing bottlenecks for wider accessibility.

Keywords:

LAMP NINA devices SARS‐CoV2 isothermal amplification phase change materials

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Last Updated: Jun 20, 2026

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Published on: November 15, 2017

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Area of Science:

Biotechnology
Medical Diagnostics
Point-of-Care Testing

Background:

Centralized diagnostic testing faces significant logistical and resource limitations, as highlighted during the SARS-CoV-2 pandemic.
Decentralized, self-administered testing offers a scalable solution to overcome these bottlenecks.
Loop-mediated amplification (LAMP) is a powerful nucleic acid amplification technique suitable for rapid diagnostics.

Purpose of the Study:

To present a novel, non-instrumental device for achieving isothermal conditions required for LAMP assays.
To enable decentralized detection of SARS-CoV-2 RNA for at-home testing.
To develop a cost-effective, environmentally friendly, and easily deployable diagnostic tool.

Main Methods:

A simple, non-instrumental device was engineered using an aluminum coffee capsule, phase change material, and a 3D-printed holder.
The device leverages boiling water to achieve and maintain a stable isothermal temperature of 65°C for 25 minutes.
This temperature is optimal for running SARS-CoV-2 RNA detection via LAMP.

Main Results:

The developed device successfully maintained the required isothermal conditions for LAMP reactions.
It demonstrated potential for cost-effective, user-friendly, and environmentally conscious SARS-CoV-2 RNA detection.
The device's design is adaptable for various LAMP-based applications beyond SARS-CoV-2.

Conclusions:

This innovative, low-cost device effectively facilitates isothermal LAMP reactions for decentralized diagnostics.
It presents a viable solution for scalable, accessible, and environmentally sustainable point-of-care testing.
The technology holds promise for widespread deployment in diverse diagnostic scenarios.