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Related Experiment Video

Updated: Sep 30, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Endoscopic en-face optical coherence tomography and fluorescence imaging using correlation-based probe tracking.

Manuel J Marques1,2, Michael R Hughes1,2, Adrián F Uceda1

  • 1Applied Optics Group, Physics and Astronomy, Division of Natural Sciences, University of Kent, Canterbury CT2 7NH, United Kingdom.

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|March 14, 2022
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Summary
This summary is machine-generated.

This study presents a novel method to create 3D optical coherence tomography (OCT) and fluorescence images using a simple 1D scanning probe. This technique enables real-time 3D visualization by manually scanning the probe, overcoming limitations of complex 2D systems.

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Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Optical Coherence Tomography

Background:

  • Forward-viewing endoscopic optical coherence tomography (OCT) enables 3D *in vivo* imaging and can be combined with fluorescence imaging.
  • Developing high-performance, miniaturized 2D scanning systems for OCT with a large field-of-view presents significant technical challenges.

Purpose of the Study:

  • To demonstrate a method for transforming a 1D scanning OCT probe into a 2D scanning probe for volumetric imaging.
  • To enable real-time 3D *en-face* OCT and fluorescence imaging by correcting for probe motion during manual scanning.

Main Methods:

  • A 1D scanning probe generating cross-sectional OCT (B-scans) and 1D fluorescence T-scans is manually scanned along a second axis to create 2D scans.
  • OCT volumes are reconstructed from B-scans using speckle decorrelation for out-of-plane motion estimation.
  • Image registration via normalized cross-correlation corrects in-plane motion within B-scans.

Main Results:

  • The system successfully generates 3D OCT volumes and fluorescence images from a manually scanned 1D probe.
  • Real-time display of motion-corrected *en-face* OCT slices and fluorescence images is achieved during scanning.
  • The method effectively handles out-of-plane motion up to 4 mm/s with a B-scan frame rate of 250 Hz.

Conclusions:

  • Manual scanning of a 1D OCT probe provides a viable alternative to complex 2D scanning systems for 3D *in vivo* imaging.
  • This approach simplifies the hardware requirements for endoscopic OCT while enabling real-time 3D visualization.
  • The developed technique offers a practical solution for enhanced endoscopic imaging in biomedical applications.