Related Experiment Video
Updated: Sep 30, 2025
In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Trigeminal Ganglia
Published on: August 1, 2025
In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals
Devora Gershon1,2, Robert H Negev-Goldstein1,2, Lama Abd Al Razzaq1,2
1Department of Medical Neurobiology, Institute for Medical Research Israel Canada, Faculty of Medicine, The Hebrew University, Jerusalem 9112001, Israel.
Abstract:
This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).
