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Updated: Sep 29, 2026

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
Published on: November 17, 2018
Protocol to assess the impact of early-life antibiotic exposure on murine longevity
Miriam A Lynn1, Feargal J Ryan1,2, Yee C Tee1
1Precision Medicine Theme, South Australian Health and Medical Research Institute, Adelaide, SA 5001, Australia.
Abstract:
We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extraction and 16S rRNA gene sequencing to longitudinally profile the composition of the fecal microbiota. Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).

