Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative faecal immunochemical tests to guide colorectal cancer pathway referral in primary care. A systematic review, meta-analysis and cost-effectiveness analysis.

Health technology assessment (Winchester, England)·2025
Same author

Analytical evaluation of eight qualitative FIT for haemoglobin products, for professional use in the UK.

Clinical chemistry and laboratory medicine·2024
Same author

The repeat FIT (RFIT) study: Does repeating faecal immunochemical tests provide reassurance and improve colorectal cancer detection?

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2024
Same author

Sample comparison of BÜHLMANN fCAL Turbo and OC-FCa faecal calprotectin methods.

Annals of clinical biochemistry·2024
Same author

A service evaluation of the use of faecal immunochemical tests in symptomatic patients aged under 50 years presenting to primary care.

Annals of clinical biochemistry·2023
Same author

Comparison and commutability study among four faecal immunochemical tests (FIT) systems.

Clinical chemistry and laboratory medicine·2023

Related Experiment Video

Updated: Sep 30, 2025

Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors
05:28

Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors

Published on: May 12, 2022

3.2K

Evaluation of a faecal calprotectin method using the OC-SENSOR PLEDIA.

Shane O'Driscoll1, Carolyn Piggott1, Sally C Benton1,2

  • 1NHS Bowel Cancer Screening Programme Southern Hub, Royal Surrey County Hospital, Guildford, UK.

Clinical Chemistry and Laboratory Medicine
|March 14, 2022
PubMed
Summary

The new OC-FCa method for faecal calprotectin (f-cal) shows good performance but a positive bias. Further clinical studies are needed to establish appropriate cut-off levels for differentiating inflammatory bowel disease.

More Related Videos

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

17.1K
In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
10:05

In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays

Published on: September 20, 2021

2.5K

Related Experiment Videos

Last Updated: Sep 30, 2025

Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors
05:28

Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors

Published on: May 12, 2022

3.2K
Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

17.1K
In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
10:05

In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays

Published on: September 20, 2021

2.5K

Area of Science:

  • Clinical Chemistry
  • Gastroenterology
  • Biomarker Analysis

Background:

  • Faecal calprotectin (f-cal) is recommended for differentiating inflammatory bowel diseases (IBD) from irritable bowel syndrome.
  • Traditional f-cal sample collection involves home-based collection into pots, followed by laboratory analysis.
  • Faecal haemoglobin (f-Hb) testing utilizes specific home-collection devices compatible with automated analyzers.

Purpose of the Study:

  • To evaluate the performance of the OC-FCa method for f-cal analysis.
  • To assess the OC-FCa method's analytical characteristics and compare it with an existing f-cal assay.
  • To determine the clinical utility of the OC-FCa method in patient sample categorization.

Main Methods:

  • Analytical performance of OC-FCa was assessed, including limit of blank, limit of detection, limit of quantification, imprecision, linearity, prozone, recovery, and carryover.
  • A method comparison was conducted between OC-FCa and the BÜHLMANN fCAL® turbo assay using patient samples and external quality assessment (EQA) materials.
  • Categorization agreement was evaluated using Cohen's Kappa statistic based on established clinical cut-off levels.

Main Results:

  • The OC-FCa method demonstrated acceptable analytical performance with a limit of quantification of 20 μg/g and high recovery (99.6%).
  • A significant positive bias was observed for OC-FCa compared to the BÜHLMANN fCAL® turbo assay (p < 0.001).
  • Substantial agreement was found for cut-off levels <50 μg/g (κ=0.80) and >150 μg/g (κ=0.63), with fair agreement in the 50-150 μg/g range (κ=0.22).

Conclusions:

  • The OC-FCa method exhibits robust analytical performance for faecal calprotectin measurement.
  • A positive bias necessitates further clinical investigation to establish validated cut-off levels for patient management.
  • Standardization and clinical validation are crucial for the widespread adoption of the OC-FCa assay in differentiating gastrointestinal conditions.