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Updated: Sep 30, 2025

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

30.1K

CTRP15 promotes macrophage cholesterol efflux and attenuates atherosclerosis by increasing the expression of ABCA1

Wei-Hua Tan1, Zheng-Liang Peng1, Ting You1

  • 1Department of Emergency Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.

Journal of Physiology and Biochemistry
|March 14, 2022
PubMed

Insights

C1q tumor necrosis factor-related protein 15 (CTRP15) reduces atherosclerosis by enhancing cholesterol removal. This myokine boosts HDL-C levels and reverse cholesterol transport, offering a potential therapeutic target for cardiovascular disease.

Area of Science:

  • Cardiovascular Biology
  • Metabolic Research
  • Molecular Medicine

Background:

  • C1q tumor necrosis factor-related protein 15 (CTRP15) is a myokine linked to cardiovascular disease.
  • The specific role of CTRP15 in atherosclerosis remains largely uncharacterized.

Purpose of the Study:

  • To elucidate the function of CTRP15 in the development of atherosclerosis.
  • To investigate the molecular mechanisms underlying CTRP15's effects on atherosclerosis.

Main Methods:

  • Apolipoprotein E-deficient (apoE-/-) mice were used to study atherosclerosis in vivo.
  • Lentivirus-mediated CTRP15 overexpression was employed in both in vivo and in vitro models.
  • Macrophage lipid accumulation and cholesterol efflux assays were performed.
  • The T-cadherin/miR-101-3p/ABCA1 pathway was analyzed to understand the mechanism.

Main Results:

  • CTRP15 overexpression significantly reduced atherosclerotic plaque burden in apoE-/- mice.
  • CTRP15 enhanced reverse cholesterol transport (RCT) efficiency and increased high-density lipoprotein cholesterol (HDL-C) levels.
  • In vitro, CTRP15 inhibited lipid accumulation and promoted cholesterol efflux from macrophages.
  • CTRP15 upregulated T-cadherin, decreased miR-101-3p, and consequently increased ABCA1 expression, facilitating cholesterol efflux.

Conclusions:

  • CTRP15 plays a protective role against atherosclerosis development.
  • CTRP15 inhibits atherosclerosis by enhancing RCT and increasing HDL-C levels through the T-cadherin/miR-101-3p/ABCA1 pathway.
  • Targeting CTRP15 presents a promising therapeutic strategy for atherosclerotic cardiovascular diseases.

Keywords:
ABCA1AtherosclerosisCTRP15Cholesterol effluxReverse cholesterol transport

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