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Updated: Sep 30, 2025

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells
Published on: November 13, 2014
Synthetic mRNA for ex vivo therapeutic applications
Hideyuki Nakanishi1, Keiji Itaka1
1Department of Biofunction Research, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan.
Abstract:
Synthetic mRNA is attracting much attention as a new drug modality. Now, there is great interest in the next applications of mRNA medicines, especially for therapeutic purposes of various diseases. Not only in vivo applicable mRNA medicines, there are many researches using ex vivo or in vitro mRNA transfection, some of which are already used in the preclinical or clinical settings. In this short article, the ex vivo mRNA applications are reviewed, in the hope of providing insight to develop future mRNA medicines.
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