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Highly sensitive fluorescent aptasensor based on MoS2 nanosheets for one-step determination of methamphetamine
Wenyan Yao1, Jiang Ling1, Wenqi Zhang1
1Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, 410013, Hunan, China.
Summary
This study introduces a new fluorescence aptasensor using MoS2 nanosheets for sensitive MTA detection. The method offers a low detection limit and successfully identifies MTA in biological samples and forensic cases.
Area of Science:
- Materials Science
- Analytical Chemistry
- Biomedical Engineering
Background:
- MTA determination is crucial for forensic science and clinical diagnostics.
- Existing methods for MTA detection may lack sensitivity or require complex procedures.
- Development of rapid, sensitive, and selective aptasensors is highly desirable.
Purpose of the Study:
- To develop a simple, sensitive, and one-step fluorescence aptasensor for MTA determination.
- To utilize MoS2 nanosheets and a FAM-labeled aptamer for enhanced sensing performance.
- To validate the aptasensor's efficacy in complex biological matrices and real forensic samples.
Main Methods:
- Fabrication of a sensing platform by adsorbing FAM-labeled aptamer onto MoS2 nanosheets.
- Utilizing the fluorescence quenching and recovery mechanism upon MTA binding.
- Characterization of the aptasensor's performance, including linearity, detection limit, and selectivity.
- Application of the aptasensor to spiked human blood, urine, and real forensic samples.
Main Results:
- The aptasensor demonstrated a linear correlation for MTA detection between 5 and 2400 nM.
- A low detection limit of 2.3 nM (S/N=3) was achieved.
- The sensor exhibited minimal cross-reactivity with common illicit drugs like ketamine, morphine, and cocaine.
- Successful application in identifying MTA in spiked biological fluids and forensic samples from a real case.
Conclusions:
- The developed MoS2-based fluorescence aptasensor provides a simple, sensitive, and selective method for MTA determination.
- This aptasensor shows great potential for practical applications in forensic identification and clinical diagnostics.
- The one-step detection strategy offers advantages in terms of speed and ease of use.
