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Mechanisms of plasmid-mediated antibiotic resistances in Vibrio parahaemolyticus

H Hasegawa, H Hamashima, T Arai

    Microbiology and Immunology
    |January 1, 1986
    PubMed

    Insights

    Mechanisms of drug resistance in Vibrio parahaemolyticus ST550 were studied. Resistance to chloramphenicol, aminoglycosides, and beta-lactams was linked to specific enzymes like CAT, AAD(3"), APH(3'), and TEM penicillinase.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Antimicrobial Resistance

    Background:

    • Clinical isolates of Vibrio parahaemolyticus (V. parahaemolyticus) can exhibit resistance to multiple antibiotics.
    • Understanding the genetic basis of this resistance is crucial for effective treatment strategies.

    Purpose of the Study:

    • To investigate the specific molecular mechanisms conferring resistance in a clinical isolate of V. parahaemolyticus ST550.
    • To identify the enzymes responsible for resistance to chloramphenicol, aminoglycoside, and beta-lactam antibiotics.

    Main Methods:

    • Phenotypic characterization of antibiotic resistance in V. parahaemolyticus ST550.
    • Molecular analysis to identify genes encoding resistance enzymes.
    • Enzyme assays to confirm the activity of identified resistance determinants.

    Main Results:

    • The V. parahaemolyticus ST550 isolate displayed resistance to chloramphenicol (CP), aminoglycoside antibiotics (AGs), and beta-lactam antibiotics.
    • Resistance to CP was attributed to chloramphenicol acetyltransferase (CAT).
    • Resistance to AGs was mediated by aminoglycoside-3"-adenylyltransferase (AAD(3")) and aminoglycoside-3 apie-phosphotransferase (APH(3")).
    • Resistance to beta-lactam antibiotics was due to TEM type penicillinase.

    Conclusions:

    • Specific enzymes, including CAT, AAD(3"), APH(3"), and TEM penicillinase, are responsible for the observed multidrug resistance in V. parahaemolyticus ST550.
    • This finding highlights the importance of identifying resistance mechanisms for guiding antibiotic therapy against V. parahaemolyticus infections.

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