Related Experiment Video
Updated: Sep 30, 2025
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
Published on: January 22, 2015
Synthesis, characterization, and biological evaluation of novel selenium-containing chitosan derivatives
Wanwen Chen1, Hao Cheng2, Lingyun Chen3
1School of Food Science and Technology, State Key Laboratory of Food Science and Technology, Jiangnan University, Jiangsu, China; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Jiangsu, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Jiangsu, China.
Abstract:
Though great efforts have been made to develop selenium polysaccharides with unique properties using HNO3-Na2SeO3 methods, the Se content is still low due to the poor esterification efficiency of H2SeO3. Herein, selenodiacetic acid (SA) was introduced into chitosan (CS) to synthesize O-selenodiacetyl chitosan (OSAC) and chitosan-ammonium selenodiacetate (CASA) by covalent and non-covalent interaction, respectively. The obtained CS derivatives were characterized by UV-vis, FTIR, 1H NMR, XPS, TGA, and XRD spectra, and the OSAC and CASA showed high Se content up to 15,720 ± 475 and 26,363 ± 698 μg/g. The OSAC and CASA demonstrated increased antioxidant activities compared to the CS in DPPH and ABTS free radical scavenging assays. Moreover, they exhibited a potent anticancer effect on HepG2 cells with the IC50 values of 0.918 and 1.459 μg/mL. Taken together, this study provides a promising strategy for the design of novel selenium polysaccharides with high Se content and greater biological activity.
