Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

B Cell Activation and Differentiation

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Asymptomatic Tuberculosis: The Silent Face of the Disease Among Migrants and Refugees and the Challenges in Brazil.

Journal of immigrant and minority health·2026

Same author

Evaluation of Antileishmanial and Antibacterial Activity of Bioconjugate Guanidine-Temporin.

Pharmaceuticals (Basel, Switzerland)·2026

Same author

Effectiveness of CoronaVac in a pioneer risk-based allocation clinical trial during the COVID-19 pandemic.

PloS one·2026

Same author

Reclone: a global research community building equitable access to reagents.

Nature methods·2026

Same author

Ancestral Protein Reconstruction Uncovers a Thermotolerant Rieske Oxygenase with Enhanced <i>O</i>-Demethylation Activity toward 3-<i>O</i>-Methylgallate.

ACS synthetic biology·2026

Same author

Guanidines: Privileged Scaffolds Against Neglected Tropical Diseases: A Review.

Pharmaceuticals (Basel, Switzerland)·2026

Search research articles

Related Experiment Video

Updated: Sep 30, 2025

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

ZIKV B-cell epitopes for immunodiagnostic tests.

Janaina Rigotti Kubiszeski¹, David José Ferreira da Silva¹, Gabriela de Lima Menezes²

¹Universidade Federal de Mato Grosso (UFMT), Instituto de Ciências da Saúde, Avenida Alexandre Ferronato, 1200, Sinop, Mato Grosso 78550-720, Brazil.

Journal of Immunological Methods

|March 15, 2022

Summary

Developing new Zika virus (ZIKV) diagnostic tools is crucial. Researchers identified specific ZIKV epitopes to improve serological test accuracy, reducing false positives from related Flaviviruses like dengue.

Keywords:

Arbovirus Bioinformatics Immunoassay Peptide

More Related Videos

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Zika Virus Specific Diagnostic Epitope Discovery

Zika Virus Specific Diagnostic Epitope Discovery

Published on: December 12, 2017

Related Experiment Videos

Last Updated: Sep 30, 2025

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Zika Virus Specific Diagnostic Epitope Discovery

Zika Virus Specific Diagnostic Epitope Discovery

Published on: December 12, 2017

Area of Science:

Virology
Immunology
Bioinformatics

Background:

Serological tests are vital for diagnosing Zika virus (ZIKV) infections and surveillance.
Cross-reactivity with other Flaviviruses, particularly dengue virus, often leads to inaccurate ZIKV test results (false positives).

Purpose of the Study:

To identify specific B-cell epitopes of ZIKV for improved diagnostic assay development.
To overcome limitations of current serological tests caused by cross-reactivity.

Main Methods:

Utilized three online bioinformatics tools to predict potential ZIKV B-cell epitopes.
Performed molecular dynamic simulations to assess epitope stability and suitability.
Synthesized two selected epitopes for further evaluation.

Main Results:

Identified and synthesized two ZIKV B-cell epitopes with favorable characteristics.
The selected epitopes demonstrated suitability for use in serological assays.
The approach aims to enhance diagnostic specificity for ZIKV.

Conclusions:

The identified ZIKV epitopes show promise for developing more accurate serological diagnostic tools.
This strategy can help differentiate ZIKV infections from other Flavivirus infections, improving surveillance and patient care.