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Updated: Sep 30, 2025

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
ZIKV B-cell epitopes for immunodiagnostic tests
Janaina Rigotti Kubiszeski1, David José Ferreira da Silva1, Gabriela de Lima Menezes2
1Universidade Federal de Mato Grosso (UFMT), Instituto de Ciências da Saúde, Avenida Alexandre Ferronato, 1200, Sinop, Mato Grosso 78550-720, Brazil.
Abstract:
The use of serological tests is valuable to diagnose Zika virus (ZIKV) infection and carry out epidemiological surveillance. However, ZIKV serological tests may result in false positives due to cross-reactivity between antibodies against other Flavivirus, especially dengue virus that worldwide disseminated. We used three online tools to predict amino acid sequences of B-cell epitopes. We selected and synthetized two epitopes that showed appropriate features in the molecular dynamic simulation and demonstrated to be suitable for serological assays.

