A novel method for miRNA detection based on target-triggered transcription of a light-up RNA aptamer
Seoyoung Lee1, Hansol Kim1, Yeonkyung Park1
1Department of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. hgpark@kaist.ac.kr.
Chemical Communications (Cambridge, England)
|March 15, 2022
Abstract:
We herein describe a novel method for miRNA detection based on target-triggered transcription of a light-up RNA aptamer (TTRApt), consequently producing a highly enhanced fluorescence signal through specific binding of a light-up RNA aptamer to the corresponding dye. Using this strategy, we successfully identified target miRNA down to 59.4 aM with excellent specificity.
