Complete Genome Sequence of Alcaligenes faecalis Phage Piluca

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Joshua Wan1, Zihao Yu1, James Clark1,2

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|March 15, 2022
PubMed

Insights

The genome of Alcaligenes faecalis phage Piluca was sequenced, revealing it is a novel temperate phage. This discovery expands our understanding of phage diversity and potential antimicrobial applications.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Alcaligenes faecalis is an opportunistic pathogen known for drug resistance.
  • Bacteriophages (phages) are viruses that infect bacteria and can be explored for therapeutic potential.

Purpose of the Study:

  • To describe the genome of the Alcaligenes faecalis phage Piluca.
  • To analyze the genetic makeup of Piluca to infer its lifestyle and evolutionary relationships.

Main Methods:

  • Whole-genome sequencing of the A. faecalis phage Piluca.
  • Bioinformatic analysis of the Piluca genome, including gene content and comparison with existing databases.

Main Results:

  • The complete genome sequence of phage Piluca (35,451 bp) was determined.
  • Piluca is genetically distinct from previously isolated phages in the NCBI database.
  • Genes encoding CI-like and Cro-like repressors and a tyrosine integrase were identified, indicative of a temperate phage.

Conclusions:

  • Phage Piluca represents a novel phage with a temperate lifestyle.
  • The genetic characteristics of Piluca provide insights into phage-bacteria interactions involving A. faecalis.
  • Further studies on Piluca may inform the development of phage-based strategies against drug-resistant A. faecalis.

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