Human periodontal ligament stem cells with distinct osteogenic potential induce bone formation in rat calvaria defects
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Updated: Sep 30, 2025
Use of Human Perivascular Stem Cells for Bone Regeneration
Published on: May 25, 2012
Leticia Faustino Adolpho1, Helena Bacha Lopes1, Gileade Pereira Freitas1
1Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Av do Café, s/n, Ribeirão Preto, SP, 14040-904, Brazil.
Abstract:
Aim: This study aimed to evaluate the ability of human periodontal ligament stem cells (PDLSCs) with high (HP-PDLSCs) and low (LP-PDLSCs) osteogenic potential, in addition to mixed cells, to repair bone tissue. Methods: Cell phenotype, proliferation and differentiation were evaluated. Undifferentiated PDLSCs were injected into rat calvarial defects and the new bone was evaluated by μCT, histology and real-time PCR. Results: PDLSCs exhibited a typical mesenchymal stem cell phenotype and HP-PDLSCs showed lower proliferative and higher osteogenic potential than LP-PDLSCs. PDLSCs induced similar bone formation and histological analysis suggests a remodeling process, confirmed by osteogenic and osteoclastogenic markers, especially in tissues derived from defects treated with HP-PDLSCs. Conclusion: PDLSCs induced similar bone formation irrespective of their in vitro osteogenic potential.
