Related Experiment Video

Updated: Sep 30, 2025

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

18.8K

Functional characterization of the human Cdk10/Cyclin Q complex

Robert Düster1, Yanlong Ji2,3,4, Kuan-Ting Pan3,4

  • 1Institute of Structural Biology, University of Bonn, Venusberg-Campus 1, 53127 Bonn, Germany.

Open Biology
|March 16, 2022
PubMed

Insights

Cyclin-dependent kinase 10 (Cdk10) regulates cell cycle and transcription. This study identifies its substrates and phosphosites, revealing its role in neural development and tumor suppression.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Cyclin-dependent kinases (CDKs) are crucial regulators of cell cycle and transcription.
  • Cdk10's role in neural development and tumor suppression is established, but its molecular mechanisms remain unclear.

Purpose of the Study:

  • To elucidate the substrate specificity and function of Cdk10.
  • To identify novel Cdk10 substrates and regulatory interactions.

Main Methods:

  • Recombinant Cdk10/CycQ protein complex used for in vitro substrate characterization.
  • Analogue-sensitive mutant kinase employed to identify in vivo phosphosites in HEK cells.
  • In vitro kinase assays to assess inhibition by CDK inhibitors and cross-phosphorylation by other CDKs.

Main Results:

  • Cdk10 phosphorylates RNA pol II CTD, c-MYC, and RB1 in vitro.
  • Identified 89 Cdk10 phosphosites on 66 proteins involved in cell cycle, translation, stress response, growth signaling, and transcriptional regulation.
  • Cdk10 is a substrate of Cdk1 and Cdk5, suggesting cross-talk between these kinases.

Conclusions:

  • Cdk10 plays a hybrid role in both cell cycle and transcriptional regulation.
  • This functional characterization provides insights into Cdk10's involvement in neural development and tumor suppression.
Keywords:
CDK10Cyclin MCyclin QRNA polymerase IIcell cycletranscription

More Related Videos

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors10:33

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors

Published on: October 26, 2015

11.4K
Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk113:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

12.0K

Related Concept Videos

Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.7K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.0K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.7K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.0K
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
3.6K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.9K