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An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
Published on: December 10, 2013
IRIS: Infection with RespIratory Syncytial Virus in infants-a prospective observational cohort study
Martin Wetzke1,2,3, Dominik Funken1, Mathias Lange4
1Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, Hanover, Germany.
Insights
The IRIS study investigates respiratory syncytial virus (RSV) in infants, aiming to identify factors influencing severe RSV infections. Understanding these factors may lead to new diagnostic and treatment strategies for infant respiratory illnesses.
Area of Science:
- Pediatric Infectious Diseases
- Virology
- Immunology
Background:
- Respiratory syncytial virus (RSV) is a leading cause of severe lower respiratory tract infections in infants globally.
- RSV infection results in millions of hospitalizations and tens of thousands of deaths annually in young children.
- The IRIS study addresses the significant public health burden of RSV in infants.
Purpose of the Study:
- To identify host and viral factors that determine the severity of RSV disease in infants.
- To enhance the understanding of the infant immune response to RSV infection.
- To uncover potential novel diagnostic markers and therapeutic targets for RSV and other infant respiratory infections.
Main Methods:
- The IRIS study is a multi-center observational study enrolling infants hospitalized with severe RSV infection and healthy controls.
- Inclusion criteria focus on infants aged 0-36 months with confirmed RSV hospitalization, excluding those with significant comorbidities.
- Data collection includes comprehensive clinical information, blood and respiratory specimens for pathogen analysis (multiplex PCR), and biobanking of various biomaterials for multi-omics characterization.
Main Results:
- This section is not available in the provided abstract.
Conclusions:
- The IRIS study's multi-omics approach in well-characterized children aims to elucidate the immune response to RSV.
- Findings are expected to advance our comprehension of RSV pathogenesis in infants.
- The study may reveal new avenues for diagnosing and treating infant respiratory infections.
Background:
Respiratory syncytial virus (RSV) is the most common cause of acute lower respiratory tract infection in infants. Globally, RSV is responsible for approximately 3.2 million hospital admissions and about 60,000 in-hospital deaths per year.
Methods:
Infection with RespIratory Syncytial Virus (IRIS) is an observational, multi-centre study enrolling infants with severe RSV infection and healthy controls. Inclusion criteria are age between 0 and 36 months and hospitalisation due to RSV infection at three German sites. Exclusion criteria are premature birth, congenital or acquired bronchopulmonary or cardiac diseases, and immunodeficiency. Healthy control probands are enrolled via recruitment of patients undergoing routine surgical procedures. Blood and respiratory specimens are collected upon admission, and RSV and other pathogens are analysed by multiplex polymerase chain reaction. Different biomaterials, including plasma, nasal lining fluid, blood cells, DNA, and RNA specimens, are sampled in a dedicated biobank. Detailed information on demographic characteristics and medical history is recorded, and comprehensive clinical data, including vital signs, medication, and interventions.
Discussion:
The IRIS study aims to discover host and viral factors controlling RSV disease courses in infants. The approach including multi-omics characterisation in clinically well-characterized children with RSV bronchiolitis seeks to improve our understanding of the immune response against this virus. It may disclose novel diagnostic and treatment approaches for respiratory infections in infants.
Trial Registration:
ClinicalTrials.gov, NCT04925310. Registered 01 October 2021-Retrospectively registered. https://clinicaltrials.gov/ct2/show/NCT04925310?cond=NCT04925310&draw=2&rank=1.
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