Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cryptococcus neoformans: pseudohyphal forms surviving culture with Acanthamoeba polyphaga.

J B Neilson, M H Ivey, G S Bulmer

    Infection and Immunity
    |April 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Human pathogenic fungi in China--emerging trends from ongoing national survey for 1986, 1996, and 2006.

    Mycopathologia·2011
    Same author

    Changes in frequency of agents of tinea capitis in school children from Western China suggest slow migration rates in dermatophytes.

    Medical mycology·2008
    Same author

    Seborrheic dermatitis flare in a Dutch male due to commensal Malassezia furfur overgrowth.

    Medical mycology·2008
    Same author

    Incidence of histoplasmin hypersensitivity in the Philippines.

    Mycopathologia·2001
    Same author

    Training medical myocologists in developing countries.

    Medical mycology·2001
    Same author

    The antifungal action of dandruff shampoos.

    Mycopathologia·2000

    Free-living amoebae, Acanthamoeba polyphaga, were observed to consume Cryptococcus neoformans. Surviving yeast developed into less virulent pseudohyphal forms, suggesting amoebae may control fungal populations in nature.

    Area of Science:

    • Microbiology
    • Mycology
    • Environmental Science

    Background:

    • Cryptococcus neoformans is a significant human pathogen.
    • The gastrointestinal tract is a potential entry route for C. neoformans.
    • The interaction between C. neoformans and environmental microorganisms is not fully understood.

    Purpose of the Study:

    • To investigate the interaction between Acanthamoeba polyphaga and Cryptococcus neoformans.
    • To determine the effect of amoebic predation on C. neoformans morphology and virulence.

    Main Methods:

    • Co-culturing C. neoformans with Acanthamoeba polyphaga.
    • Microscopic examination of amoebic trophozoites for yeast cells.
    • Incubation and observation of surviving C. neoformans for morphological changes.

    Related Experiment Videos

  • Virulence studies of C. neoformans isolates in mice after intracranial inoculation.

    • Main Results:

    • Acanthamoeba polyphaga ingested and killed a significant number of Cryptococcus neoformans cells.
    • Surviving C. neoformans developed atypical pseudohyphal forms.
    • Pseudohyphal isolates from seven of eight strains showed reduced virulence in mice.
    • Amoebic predation led to the emergence of less virulent C. neoformans strains.

    Conclusions:

    • Acanthamoeba polyphaga can significantly reduce C. neoformans populations.
    • Amoebic interaction induces morphological changes in C. neoformans, leading to decreased virulence.
    • Soil amoebae may play a role in regulating environmental C. neoformans populations.