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Circ_0060531 knockdown ameliorates IL-22-induced keratinocyte damage by binding to miR-330-5p to decrease GAB1

Quan Shi1, Jing Luo1, Weiming Chen1

  • 1Department of Dermatology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan City, China.

Autoimmunity
|March 16, 2022
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Summary
This summary is machine-generated.

Circular RNA (circRNA) circ_0060531 exacerbates psoriasis by targeting the microRNA-330-5p/GAB1 pathway. Inhibiting circ_0060531 offers a potential therapeutic strategy for psoriasis by reducing keratinocyte dysfunction.

Keywords:
GAB1Psoriasiscirc_0060531miR-330-5p

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Area of Science:

  • Dermatology
  • Molecular Biology
  • RNA Biology

Background:

  • Psoriasis is a chronic, immune-mediated skin condition.
  • Circular RNAs (circRNAs) are implicated in psoriasis pathogenesis.
  • The specific role of circ_0060531 in psoriasis remains unclear.

Purpose of the Study:

  • To investigate the role of circ_0060531 in the development of psoriasis.
  • To elucidate the underlying molecular mechanism involving circ_0060531, microRNA-330-5p (miR-330-5p), and GRB2 associated binder 1 (GAB1).

Main Methods:

  • Constructed a psoriasis cell model using HaCaT cells stimulated with interleukin 22 (IL-22).
  • Quantified expression levels of circ_0060531, miR-330-5p, and GAB1 using quantitative real-time polymerase chain reaction.
  • Assessed the functional impact of circ_0060531 on IL-22-induced keratinocyte injury through various assays and analyzed protein expression via Western blot.
  • Investigated molecular interactions using dual-luciferase reporter and RNA immunoprecipitation assays.

Main Results:

  • Circ_0060531 and GAB1 expression were elevated, while miR-330-5p was reduced in psoriatic tissues and IL-22-treated cells.
  • Knockdown of circ_0060531 alleviated IL-22-induced keratinocyte proliferation, migration, and inflammation.
  • Circ_0060531 functioned as a sponge for miR-330-5p, regulating IL-22-treated HaCaT cells.
  • MiR-330-5p mediated IL-22-induced HaCaT cell damage by targeting GAB1, and circ_0060531 modulated GAB1 via miR-330-5p.

Conclusions:

  • Circ_0060531 knockdown mitigates IL-22-induced keratinocyte dysfunction.
  • The miR-330-5p/GAB1 pathway is crucial in the mechanism.
  • Circ_0060531 represents a potential therapeutic target for psoriasis treatment.