Related Experiment Video

Updated: Sep 30, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.5K

Circ_0060531 knockdown ameliorates IL-22-induced keratinocyte damage by binding to miR-330-5p to decrease GAB1

Quan Shi1, Jing Luo1, Weiming Chen1

  • 1Department of Dermatology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan City, China.

Autoimmunity
|March 16, 2022
PubMed
Abstract

Insights

Circular RNA (circRNA) circ_0060531 exacerbates psoriasis by targeting the microRNA-330-5p/GAB1 pathway. Inhibiting circ_0060531 offers a potential therapeutic strategy for psoriasis by reducing keratinocyte dysfunction.

Area of Science:

  • Dermatology
  • Molecular Biology
  • RNA Biology

Background:

  • Psoriasis is a chronic, immune-mediated skin condition.
  • Circular RNAs (circRNAs) are implicated in psoriasis pathogenesis.
  • The specific role of circ_0060531 in psoriasis remains unclear.

Purpose of the Study:

  • To investigate the role of circ_0060531 in the development of psoriasis.
  • To elucidate the underlying molecular mechanism involving circ_0060531, microRNA-330-5p (miR-330-5p), and GRB2 associated binder 1 (GAB1).

Main Methods:

  • Constructed a psoriasis cell model using HaCaT cells stimulated with interleukin 22 (IL-22).
  • Quantified expression levels of circ_0060531, miR-330-5p, and GAB1 using quantitative real-time polymerase chain reaction.
  • Assessed the functional impact of circ_0060531 on IL-22-induced keratinocyte injury through various assays and analyzed protein expression via Western blot.
  • Investigated molecular interactions using dual-luciferase reporter and RNA immunoprecipitation assays.

Main Results:

  • Circ_0060531 and GAB1 expression were elevated, while miR-330-5p was reduced in psoriatic tissues and IL-22-treated cells.
  • Knockdown of circ_0060531 alleviated IL-22-induced keratinocyte proliferation, migration, and inflammation.
  • Circ_0060531 functioned as a sponge for miR-330-5p, regulating IL-22-treated HaCaT cells.
  • MiR-330-5p mediated IL-22-induced HaCaT cell damage by targeting GAB1, and circ_0060531 modulated GAB1 via miR-330-5p.

Conclusions:

  • Circ_0060531 knockdown mitigates IL-22-induced keratinocyte dysfunction.
  • The miR-330-5p/GAB1 pathway is crucial in the mechanism.
  • Circ_0060531 represents a potential therapeutic target for psoriasis treatment.
Keywords:
GAB1Psoriasiscirc_0060531miR-330-5p

More Related Videos

Demonstration of the Rat Ischemic Skin Wound Model08:35

Demonstration of the Rat Ischemic Skin Wound Model

Published on: April 1, 2015

21.6K
Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues08:06

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Published on: July 8, 2020

5.7K

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.2K