Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Response Surface Methodology01:16

Response Surface Methodology

308
Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable.
The process of RSM involves several key steps:
308
Wood Surfacing01:14

Wood Surfacing

159
Wood surfacing is a critical finishing process designed to smoothen the wood surface, enhance its dimensional accuracy, and make handling safer. This process compensates for potential shrinkage during the seasoning phase by marginally increasing the wood dimensions before surfacing. It also helps correct some distortions that may occur as the wood dries.
The equipment used in the surfacing process is a plane equipped with rotating blades. This tool efficiently smoothens the wood surface and can...
159
Design Example: Calculating Safe Diameter for Wind-Exposed Disc01:17

Design Example: Calculating Safe Diameter for Wind-Exposed Disc

193
Assessing safety in wind-exposed installations is crucial to preventing potential failures. This example explores the calculation and design adjustments needed to mount a circular disc on a building facade, where wind forces are a primary concern. A 4-meter diameter disc was initially designed as an aesthetic feature facing winds at a velocity of 25 meters per second, with an air density of 1.25 kilograms per cubic meter. Given these conditions, the drag force on the disc was determined using...
193
Design Example: Traverse Angle Computations01:25

Design Example: Traverse Angle Computations

155
Traverse angle computations are a critical component of surveying, used to compute the internal angles within a closed traverse. A traverse consists of a series of connected lines forming a closed loop, often used for land boundary delineation or mapping. Calculating the internal angles ensures accuracy in the traverse geometry and is essential for checking survey data integrity.The process begins with known azimuths and bearings of the traverse sides. Internal angles at each vertex are...
155
Turbulent Flow: Problem Solving01:09

Turbulent Flow: Problem Solving

200
Carbonation is a process used to dissolve carbon dioxide gas in a liquid, commonly used in the production of carbonated beverages. Achieving efficient carbonation requires careful control of temperature, pressure, and flow conditions. By adjusting these parameters, carbonation efficiency can be maximized, producing a higher concentration of CO2 in the liquid.
Temperature is a key factor in CO2 solubility. In this case, the CO2 gas and the liquid are cooled to 20°C. Lower temperatures...
200
Design Example: Application of Archimedes' Principle01:11

Design Example: Application of Archimedes' Principle

342
Archimedes' principle is fundamental in analyzing the buoyant force and stability of floating bodies. In this example, a wooden block with a rectangular section floats in seawater. Based on the block's dimensions, its specific gravity and the specific weight of seawater are used to find the volume of water displaced and the center of buoyancy.
The volume of seawater displaced by the block is determined by first calculating the block's weight. This is done by multiplying the...
342

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The mutational landscape of STING-induced immunity.

Nature·2026
Same author

Deep learning-enabled scaffolding of spatial arrays of PfCSP epitopes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Drug-controlled CAR T cells through the regulation of cell-cell interactions.

Nature chemical biology·2026
Same author

Mapping targetable sites on the human surfaceome for the design of novel binders.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Accurate single-domain scaffolding of three nonoverlapping protein epitopes using deep learning.

Nature chemical biology·2025
Same author

Conditional deep learning model reveals translation elongation determinants during amino acid deprivation.

Communications biology·2025
Same journal

Detection, communication, and individual identification with deep audio embeddings: A case study with North Atlantic right whales.

PLoS computational biology·2026
Same journal

Exploring the structural lexicon of the Proteome via Metric Geometry.

PLoS computational biology·2026
Same journal

Linking retinal sampling in neural encoding models to temporal profiles of visual processing in humans.

PLoS computational biology·2026
Same journal

CAdir: Joint clustering of cells and genes for single-cell transcriptomics with visualization-driven cluster quality assessment.

PLoS computational biology·2026
Same journal

Systematic design of auxotrophic strains and media conditions to probe metabolic functions in E. coli.

PLoS computational biology·2026
Same journal

Neuronal excitability and parameter variability in the Hodgkin-Huxley model.

PLoS computational biology·2026
See all related articles

Related Experiment Video

Updated: Sep 30, 2025

Structural Design and Manufacturing of a Cruiser Class Solar Vehicle
14:57

Structural Design and Manufacturing of a Cruiser Class Solar Vehicle

Published on: January 30, 2019

14.0K

RosettaSurf-A surface-centric computational design approach.

Andreas Scheck1,2, Stéphane Rosset1,2, Michaël Defferrard3

  • 1Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Plos Computational Biology
|March 16, 2022
PubMed
Summary
This summary is machine-generated.

RosettaSurf is a new computational method for protein engineering. It designs proteins by optimizing their surface shape and electrostatics, enabling the creation of novel protein functions and epitope mimics.

More Related Videos

Rendering SiO2/Si Surfaces Omniphobic by Carving Gas-Entrapping Microtextures Comprising Reentrant and Doubly Reentrant Cavities or Pillars
08:02

Rendering SiO2/Si Surfaces Omniphobic by Carving Gas-Entrapping Microtextures Comprising Reentrant and Doubly Reentrant Cavities or Pillars

Published on: February 11, 2020

9.1K
Manufacturing, Control, and Performance Evaluation of a Gecko-Inspired Soft Robot
07:40

Manufacturing, Control, and Performance Evaluation of a Gecko-Inspired Soft Robot

Published on: June 10, 2020

14.8K

Related Experiment Videos

Last Updated: Sep 30, 2025

Structural Design and Manufacturing of a Cruiser Class Solar Vehicle
14:57

Structural Design and Manufacturing of a Cruiser Class Solar Vehicle

Published on: January 30, 2019

14.0K
Rendering SiO2/Si Surfaces Omniphobic by Carving Gas-Entrapping Microtextures Comprising Reentrant and Doubly Reentrant Cavities or Pillars
08:02

Rendering SiO2/Si Surfaces Omniphobic by Carving Gas-Entrapping Microtextures Comprising Reentrant and Doubly Reentrant Cavities or Pillars

Published on: February 11, 2020

9.1K
Manufacturing, Control, and Performance Evaluation of a Gecko-Inspired Soft Robot
07:40

Manufacturing, Control, and Performance Evaluation of a Gecko-Inspired Soft Robot

Published on: June 10, 2020

14.8K

Area of Science:

  • Computational biology
  • Protein engineering
  • Structural biology

Background:

  • Proteins are often represented by atomic coordinates, but their continuous surfaces dictate function.
  • Protein surface shape and electrostatics are key to molecular interactions and function.
  • Existing protein design methods primarily rely on energy scoring functions.

Purpose of the Study:

  • To introduce RosettaSurf, a novel surface-centric computational design protocol for protein engineering.
  • To enable the design of functional sites in proteins, even without similar structural templates.
  • To explore the potential of surface properties independent of sequence and backbone configuration.

Main Methods:

  • RosettaSurf explicitly optimizes molecular surface features (shape and electrostatics) during sequence design.
  • The protocol integrates surface feature optimization with a global scoring function.
  • It diverges from traditional methods that solely use energy scoring functions.

Main Results:

  • RosettaSurf was benchmarked on sequence recovery datasets, demonstrating its efficacy.
  • The protocol successfully generated biochemically validated epitope mimics.
  • The study highlights the potential of surface-centric design for creating functional proteins.

Conclusions:

  • Explicit optimization of protein surface features offers a new paradigm for protein design.
  • RosettaSurf provides a unique approach to engineer protein function by focusing on molecular surfaces.
  • This method could lead to novel applications in protein engineering and drug discovery.