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Related Experiment Videos

Characterization of plasmids that encode for the K88 colonization antigen.

P L Shipley, C L Gyles, S Falkow

    Infection and Immunity
    |May 1, 1978
    PubMed
    Summary

    Porcine enteropathogenic Escherichia coli (EEC) virulence factors, K88 antigen and raffinose fermentation, are often encoded on a single plasmid. Deletions can result in plasmids with only raffinose fermentation genes.

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    Area of Science:

    • Microbiology
    • Genetics
    • Molecular Biology

    Background:

    • Porcine enteropathogenic Escherichia coli (EEC) poses a significant threat to animal health.
    • The K88 antigen is a critical virulence factor in EEC, contributing to disease pathogenesis.
    • Raffinose fermentation is another characteristic associated with certain EEC strains.

    Purpose of the Study:

    • To isolate and characterize plasmids encoding both K88 antigen and raffinose fermentation in EEC.
    • To understand the genetic basis and transferability of these traits.
    • To investigate plasmid variability and potential gene deletion events.

    Main Methods:

    • Plasmid isolation from multiple EEC strains.
    • Plasmid characterization using molecular techniques.
    • Analysis of gene content and size.
    • Conjugation experiments to assess transferability.

    Main Results:

    • In most EEC strains, K88 antigen and raffinose fermentation genes reside on a conserved, nonconjugative plasmid (~50 x 10^6 daltons).
    • Recombination with transfer factors can lead to larger, conjugative plasmids.
    • Plasmid deletions can result in the loss of K88 antigen genes, retaining only raffinose fermentation capability.

    Conclusions:

    • The K88 antigen and raffinose fermentation traits in EEC are frequently linked on a single plasmid.
    • Plasmid structure is relatively conserved but can undergo recombination and deletion.
    • Understanding these plasmid dynamics is crucial for controlling EEC virulence and spread.

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