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Published on: February 14, 2016
Depletion of SMARCB1 and BRD7, two SWI/SNF chromatin remodelling complex subunits, differentially impact porcine
Yu-Chun Tseng1, Jennifer S Crodian1, Ryan Cabot1
1Department of Animal Sciences, Purdue University, 270 South Russell Street, West Lafayette, IN 47907, USA.
Context:
SWI/SNF chromatin remodelling complexes are composed of multiple protein subunits and can be categorised into three sub-families, including the BAF, PBAF, and GBAF complexes. We hypothesised that depletion of SMARCB1 and BRD7, two subunits unique to different SWI/SNF sub-families, would differentially impact porcine embryo development.
Aim:
The aim of these experiments was to determine the developmental requirements of two SWI/SNF subunits, SMARCB1 and BRD7.
Methods:
RNA interference assays were used to determine the developmental requirements of SMARCB1 and BRD7 in porcine embryos.
Key Results:
Our findings indicate that knockdown of SMARCB1 dramatically reduces embryo developmental potential, with few embryos developing beyond the pronuclear stage. The knockdown of BRD7 had a less severe impact on developmental potential.
Conclusions:
Our findings also demonstrate that knockdown of SMARCB1 alters the expression of NANOG and POU5F1 (also referred to as OCT4 ).
Implications:
These findings highlight the unique developmental requirements for sub-families of SWI/SNF chromatin remodelling complexes. This new knowledge will enable us to determine how discrete genomic loci are differentially remodelled during key points in embryo development.
