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[Experience in isolating Campylobacter jejuni from patients with acute intestinal diseases]

L B Khazenson, N V Safonova, Z N Matveeva

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |July 1, 1986
    PubMed

    Insights

    Erythrite agar supplemented with sheep blood and antibiotics effectively cultivates and isolates Campylobacter jejuni (C. jejuni). This method identified C. jejuni in 10% of patients with acute infectious intestinal diseases.

    Area of Science:

    • Microbiology
    • Clinical Diagnostics
    • Medical Bacteriology

    Background:

    • Campylobacter jejuni (C. jejuni) is a significant cause of bacterial gastroenteritis.
    • Accurate isolation and cultivation of C. jejuni are crucial for diagnosis and treatment.
    • Existing culture media may require optimization for efficient C. jejuni recovery.

    Purpose of the Study:

    • To evaluate the efficacy of Erythrite agar for C. jejuni cultivation.
    • To determine the utility of Erythrite agar supplemented with blood and antibiotics for C. jejuni isolation from clinical samples.
    • To assess the prevalence of C. jejuni in patients with acute infectious intestinal diseases using the optimized medium.

    Main Methods:

    • Cultivation of C. jejuni on Erythrite agar.
    • Supplementation of Erythrite agar with hemolized sheep blood.
    • Addition of antibiotics to Erythrite agar for selective isolation.
    • Application of the optimized medium for C. jejuni isolation from patient fecal samples.

    Main Results:

    • Erythrite agar is suitable for C. jejuni cultivation when supplemented with hemolized sheep blood.
    • The addition of antibiotics to Erythrite agar enables selective isolation of C. jejuni from feces.
    • C. jejuni was successfully isolated from the feces of 10% of hospitalized patients with acute infectious intestinal diseases.

    Conclusions:

    • Optimized Erythrite agar provides an effective method for C. jejuni cultivation and isolation.
    • This medium is valuable for diagnosing C. jejuni infections in patients with gastrointestinal illness.
    • The study highlights the prevalence of C. jejuni in a specific patient cohort.

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