Related Experiment Video

Updated: Sep 30, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.2K

Complete Genome Sequences of Six Chi-Like Bacteriophages That Infect Proteus and Klebsiella

Hunter K Cobbley1, Seth I Evans1, Hannah M F Brown1

  • 1Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, USA.

Microbiology Resource Announcements
|March 17, 2022
PubMed

Insights

This study announces the full genome sequences of six Chi-like bacteriophages targeting opportunistic pathogens Proteus mirabilis and Klebsiella aerogenes. Understanding these phages aids in combating healthcare-associated infections.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Proteus mirabilis and Klebsiella aerogenes are Gram-negative opportunistic pathogens.
  • These bacteria cause significant nosocomial and healthcare-associated infections, particularly urinary tract infections.

Purpose of the Study:

  • To announce the full genome sequences of six novel Chi-like bacteriophages.
  • To contribute to the understanding of Chi-like phages and their hosts.

Main Methods:

  • Whole genome sequencing of six bacteriophages.
  • Bioinformatic analysis of phage genomes.

Main Results:

  • The complete genome sequences of five Chi-like Proteus phages (DanisaurMW, DoubleBarrel, Inception, Jing313, NotEvenPhaged) and one Klebsiella phage (Phraden) were determined.
  • These sequences provide valuable genetic information about Chi-like phages.

Conclusions:

  • The genomic data advances the knowledge of Chi-like bacteriophages.
  • This research supports potential phage therapy development against Proteus and Klebsiella infections.

More Related Videos

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.5K
Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

12.3K

Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
195
Bacterial Phylum Chlamydiae01:29

Bacterial Phylum Chlamydiae

The phylum Chlamydiae or Chlamydiota is composed of a single order, Chlamydiales. This phylum consists entirely of obligate intracellular parasites that infect eukaryotic hosts. While human pathogens within this group have been studied extensively, the phylum encompasses many species capable of interacting with various eukaryotic organisms. Members of Chlamydiae are typically small cocci, approximately 0.5 μm in diameter, and exhibit a distinctive developmental cycle. As is characteristic...
138
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
352
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
63.7K
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
72.6K
Bacterial Phylum Proteobacteria01:26

Bacterial Phylum Proteobacteria

Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
197